Antibody:	anti-COUP-TFII (PP-H7147-00)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Strong		2B
TS21: lateral ganglionic eminence	Absent		2B
TS21: medial ganglionic eminence	Absent		2B
TS21: cerebral cortex	Moderate	Regionally restricted	2B	A moderate signal was observed in the caudal cortex.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: telencephalon	neuron of the forebrain	Present	Regionally restricted	3H , 3M , 3D , 3I , 3L , 3F , 3E , 3A , 3K , 3B	Expression was detected in a migratory stream from the caudal ganglionic eminence to the caudal cortex and hippocampus. A majority of the migrating neurons were positive for both Nr2f2 and Calb1 (71% of the Calb1+ cells).
TS21: caudal ganglionic eminence	neuron of the forebrain	Strong		3D , 3B , 3F , 3E , 3A	Many of the immunopositive cells were also positive for Calb1.
TS21: cerebral cortex	neuron of the forebrain	Present	Regionally restricted	3I , 3L , 3K , 3A , 3M , 3H	Expression was detected in the caudal cortex. Labeling weakened after the migrating cells entered the caudal cortex, but continued to be co-localized with Calb1 (56.8% of the Calb1+ cells).

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Present	Non-Uniform	6E , 6H , 6A , 6G , 6D , 6B	In the caudal part, the majority of Calb1-positive cells were also positive for Nr2f2 (64.4%). In the rostral part, most of the Calb1-positive cells were negative for Nr2f2 (only 14.4% of cells were co-labeled).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: visual cortex		Present	Non-Uniform	8B , 8A	Expression was detected in both the deep layers and the superficial layers.
TS28: visual cortex	stellate neuron	Strong	Regionally restricted	8D , 8B , 8A , 8E	Some of the upper layer cells were co-labeled with Calb2.
TS28: visual cortex	bipolar neuron	Weak	Regionally restricted	8D , 8A , 8B , 8E	Some of the upper layer cells were co-labeled with Calb2.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Moderate	Regionally restricted	S1E , S1G	Signal was detected at the border between the medial and lateral ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS21: caudal ganglionic eminence	Strong		S1F

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Weak	Regionally restricted	S2C , S2B , S2A	A large portion of Calb1+ cells in the caudal cortex co-stain weakly for Nr2f2.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: visual cortex	cortical interneuron	Present	Regionally restricted	S5E , S5B , S5J , S5I , S5F , S5A	Expression was detected in interneurons in the P25 visual cortex. Some immunopositive cells were also labeled with Sst and/or Calb2. Single positive cells were observed in the deep layers.
TS28: visual cortex	stellate neuron	Present	Regionally restricted	S5F , S5A , S5E , S5B	Signal was observed in multipolar/stellate double-positive Calb2- and Sst-positive cells in the superficial layers.

Structure	Level	Pattern	Image	Note
TS28: visual cortex	Present	Regionally restricted	S6D , S6A , S6E , S6B	Expression was detected in the visual cortex but was not co-localized with Pvalb.