Antibody:	anti-Int6 #20
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Fixative was 2.5% paraformaldehyde/0.1% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS17: migrating neural crest	Strong	Regionally restricted	7A	Expression is in migrating neural crest emigrating from the spinal cord.
TS17: future spinal cord	Weak		7A

Structure	Level	Pattern	Image	Note
TS19: migrating neural crest	Strong	Regionally restricted	7C	Expression is in migrating neural crest emigrating from the spinal cord.
TS19: future spinal cord	Weak		7C

Structure	Cell Type	Level	Pattern	Image	Note
TS20: notochord		Strong		7E
TS20: vertebral cartilage condensation	chondrocyte	Strong	Regionally restricted	7E	Expression is perinuclear in condensing chondrocytes.

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Strong		7F

Structure	Cell Type	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	chondrocyte	Strong	Regionally restricted	7G	Expression is perinuclear in condensing chondrocytes.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Strong		7H , 7H'' , 7H'

Structure	Level	Pattern	Image	Note
TS15: embryo	Weak	Ubiquitous		The authors state that at all ages examined (E9.5-E14.5) there was a low level of expression in most tissues.

Structure	Level	Pattern	Image	Note
TS17: embryo	Weak	Ubiquitous		The authors state that at all ages examined (E9.5-E14.5) there was a low level of expression in most tissues.

Structure	Level	Pattern	Image	Note
TS19: embryo	Weak	Ubiquitous		The authors state that at all ages examined (E9.5-E14.5) there was a low level of expression in most tissues.

Structure	Level	Pattern	Image	Note
TS20: embryo	Weak	Ubiquitous		The authors state that at all ages examined (E9.5-E14.5) there was a low level of expression in most tissues.

Structure	Level	Pattern	Image	Note
TS22: embryo	Weak	Ubiquitous		The authors state that at all ages examined (E9.5-E14.5) there was a low level of expression in most tissues.