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Gene Expression Data
Assay Details
Assay
Reference: J:275851 Harris C, et al., Conversion of random X-inactivation to imprinted X-inactivation by maternal PRC2. Elife. 2019 Apr 2;8:e44258
Assay type: RNA in situ
MGI Accession ID: MGI:7495948
Gene symbol: Rlim
Gene name: ring finger protein, LIM domain interacting
Probe: Rnf12 probe
Probe preparation: Antisense labelled with fluorescein RNA
Results
Specimen 3D fl/fl: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Rlim; gray - H3K27me3.
Structure Level Pattern Image Note
TS4: blastocyst Present 3D fl/fl Rnf12 , 3D right fl/fl Rnf12
Specimen 3D -/-: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Rlim; gray - H3K27me3.
Structure Level Pattern Image Note
TS4: blastocyst Present 3D -/- Rnf12 , 3D right -/- Rnf12 Similar as in the control.
Specimen 3-S1D fl/fl male: embryonic day 3.5; Eedtm1c(EUCOMM)Wtsi/Eedtm1c(EUCOMM)Wtsi (more )
Note: Double labeled: red - Rlim; gray - H3K27me3.
Structure Level Pattern Image Note
TS4: blastocyst Present 3-S1D fl/fl Rnf12 , 3-S1D fl/fl Rnf12 right
Specimen 3-S1D fl/- or -/- male: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/? (more )
Note: Tg(Prm-cre)58Og was used to delete the Eed floxed allele in the male germline. Double labeled: red - Rlim; gray - H3K27me3.
Structure Level Pattern Image Note
TS4: blastocyst Present 3-S1D fl/- or -/- Rnf12 right , 3-S1D fl/- or -/- Rnf12 Similar as in the fl/fl controls.
Specimen 5A m-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw was used to delete the Eed floxed allele in the female germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5A upper right , 5A upper right enlarged Most nuclei displayed monoallelic expression. No expression in a subset displayed Xist RNA coating of both X-chromosomes.
Specimen 5A mz-/- female: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5A lower right enlarged , 5A lower right Most nuclei displayed monoallelic expression. No expression in a subset displayed Xist RNA coating of both X-chromosomes.
Specimen 5B mz-/- male: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eed+ (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in the female germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5B upper right , 5B upper right enlarged Expression was lost in those Xist RNA-coated X-chromosome.
Specimen 5B mz-/- male delayed: embryonic day 3.5; Eedtm1d(EUCOMM)Wtsi/Eedtm1d(EUCOMM)Wtsi (more )
Note: Tg(Zp3-cre)93Knw and Tg(Prm-cre)58Og were used to delete the Eed floxed allele in both the female and male germline.
Structure Level Pattern Image Note
TS4: blastocyst Present 5B lower right , 5B lower right enlarged Expression was lost in those Xist RNA-coated X-chromosome.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory