About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:40032 Dou C, et al., TWH regulates the development of subsets of spinal cord neurons. Neuron. 1997 Apr;18(4):539-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7488703
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-C-Isl-1 protein (K5)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 4A: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Cervical level. Double labeled: brown - Isl1 and Isl2; blue - Foxb1 reporter (assay MGI:7488687).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord cervical region spinal cord motor neuron Present Regionally restricted 4A Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4A Expressed in medial and lateral motor columns.
Specimen 4B: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Thoracic level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4B Expressed in medial motor column.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4B Expressed in medial motor column.
Specimen 4C: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Upper lumbar level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 4C Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4C Expressed in medial and lateral motor columns.
Specimen 4D: embryonic day 13.5; Foxb1tm1Sli/Foxb1+ (more )
Note: Mid lumbar level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Level Pattern Image Note
TS21: spinal cord lumbar region Present Regionally restricted 4D Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn Present Regionally restricted 4D Expressed in medial and lateral motor columns.
Specimen 4E: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Cervical level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord cervical region spinal cord motor neuron Present Regionally restricted 4E Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4E Expressed in medial and lateral motor columns.
Specimen 4F: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Thoracic level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord thoracic region spinal cord motor neuron Present Regionally restricted 4F Expressed in medial motor column.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4F Expressed in medial motor column.
Specimen 4G: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Upper lumbar level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 4G Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4G Expressed in medial and lateral motor columns.
Specimen 4H: embryonic day 13.5; Foxb1tm1Sli/Foxb1tm1Sli (more )
Note: Mid lumbar level. Triple labeled: - Isl1; blue - Foxb1 reporter (assay MGI:7488687); brown - Isl2 (assay MGI:7490072).
Structure Cell Type Level Pattern Image Note
TS21: spinal cord lumbar region spinal cord motor neuron Present Regionally restricted 4H Expressed in medial and lateral motor columns.
TS21: spinal cord ventral horn spinal cord motor neuron Present Regionally restricted 4H Expressed in medial and lateral motor columns.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/28/2024
MGI 6.13 		The Jackson Laboratory