Gene Expression Data
Assay Details
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Assay
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Reference:
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J:333946
Hattori Y, et al., CD206(+) macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia. Cell Rep. 2023 Feb 7;42(2):112092
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7488357
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Gene symbol:
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P2ry12
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Gene name:
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purinergic receptor P2Y, G-protein coupled 12
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Results
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Specimen
1A E12.5 P2RY12:
embryonic day 12.5; Cx3cr1tm1Litt/Cx3cr1 +
(more  )
Note: Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
Specimen
1A E12.5 P2RY12:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
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Specimen
1A E13.5 P2RY12:
embryonic day 13.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
Specimen
1A E13.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: pallium |
microglial cell |
Present |
Regionally restricted |
1A E13.5 P2RY12
,
1A E13.5 P2RY12/CD206
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Expression in cells intermingled with Mrc1-expressing cells. Cells co-expressing Mrc1 frequently detected at E12.5 and E13.5.
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Specimen
1A E14.5 P2RY12:
embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
Specimen
1A E14.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: pallium |
microglial cell |
Present |
Regionally restricted |
1A E14.5 P2RY12/CD206
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Expression in cells intermingled with Mrc1-expressing cells.
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Specimen
S1A E14.5 P2RY12:
embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
Specimen
S1A E14.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: pallium |
microglial cell |
Present |
Regionally restricted |
S1A
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Expression in cells intermingled with Mrc1-expressing cells.
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Specimen
S1B E15.5 P2RY12:
embryonic day 15.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
Specimen
S1B E15.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: pallium |
microglial cell |
Present |
Regionally restricted |
S1B E15.5 P2RY12/CD206
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Expression in cells intermingled with Mrc1-expressing cells.
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Specimen
S1B E16.5 P2RY12:
embryonic day 16.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
Specimen
S1B E16.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; red - Mrc1 (assay MGI:7488358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: pallium |
microglial cell |
Present |
Regionally restricted |
S1B E16.5 P2RY12/CD206
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Expression in cells intermingled with Mrc1-expressing cells.
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Specimen
S1G E12.5 P2RY12:
embryonic day 12.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
Specimen
S1G E12.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: pallium |
microglial cell |
Present |
Regionally restricted |
S1G E12.5 P2RY12/SiglecH
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Almost all P2ry12-expressing cells were positive for Siglech expression.
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Specimen
S1G E13.5 P2RY12:
embryonic day 13.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
Specimen
S1G E13.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: pallium |
microglial cell |
Present |
Regionally restricted |
S1G E13.5 P2RY12/SiglecH
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Almost all P2ry12-expressing cells were positive for Siglech expression.
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Specimen
S1G E14.5 P2RY12:
embryonic day 14.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
Specimen
S1G E14.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: pallium |
microglial cell |
Present |
Regionally restricted |
S1G E14.5 P2RY12/SiglecH
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Almost all P2ry12-expressing cells were positive for Siglech expression.
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Specimen
S1G E15.5 P2RY12:
embryonic day 15.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
Specimen
S1G E15.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: pallium |
microglial cell |
Present |
Regionally restricted |
S1G E15.5 P2RY12/SiglecH
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Almost all P2ry12-expressing cells were positive for Siglech expression.
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Specimen
S1G E16.5 P2RY12:
embryonic day 16.5; Cx3cr1tm1Litt/Cx3cr1 +
(more )
Note: Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
Specimen
S1G E16.5 P2RY12:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * ICR
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Mutant Allele(s):
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Cx3cr1tm1Litt/Cx3cr1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - P2ry12; magenta - Siglech (assay MGI:7488360).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: pallium |
microglial cell |
Present |
Regionally restricted |
S1G E16.5 P2RY12/SiglecH
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Almost all P2ry12-expressing cells were positive for Siglech expression.
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Analysis Tools