Col1a2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:331874 Lee H, et al., Deletion of ATAD3A inhibits osteogenesis by impairing mitochondria structure and function in pre-osteoblast. Dev Dyn. 2022 Dec;251(12):1982-2000
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7488025
Gene symbol:	Col1a2
Gene name:	collagen, type I, alpha 2

Antibody:	anti- Col1a1 (C7510-14)
Detection system:	Secondary antibody coupled to Cy3

Results

1If ©

Specimen If: postnatal day 0 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: femur	osteoblast	Present	Regionally restricted	1If	Expression is in osteoblasts.

1Il ©

Specimen Il: postnatal day 0; Atad3a^{tm1a(KOMP)Wtsi}/Atad3a^{tm1a(KOMP)Wtsi}, Twist2^{tm1.1(cre)Dor}/Twist2⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: femur	osteoblast	Weak		1Il

2Cc ©

Specimen 2Cc: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: femur	Present		2Cc

2Cg ©

Specimen 2Cg: postnatal day 0; Atad3a^{tm1a(KOMP)Wtsi}/Atad3a^{tm1a(KOMP)Wtsi}, Tg(Col2a1-cre)1Bhr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: femur	Present		2Cg	Expression in this mutant is the same as in controls.

2 cont'd_Ia ©

Specimen 2Ia: embryonic day 18.5; Atad3a^{tm1a(KOMP)Wtsi}/Atad3a⁺, Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: femur	Present		2 cont'd_Ia	Trabecular bone.

2 cont'd_Ie ©

Specimen 2Ie: embryonic day 18.5; Atad3a^{tm1a(KOMP)Wtsi}/Atad3a^{tm1a(KOMP)Wtsi}, Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: femur	Weak		2 cont'd_Ie	Trabecular bone.

2 cont'd_Ic ©

Specimen 2Ic: embryonic day 18.5; Atad3a^{tm1a(KOMP)Wtsi}/Atad3a⁺, Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: femur	Present		2 cont'd_Ic	Cortical bone.

2 cont'd_Ig ©

Specimen 2Ig: embryonic day 18.5; Atad3a^{tm1a(KOMP)Wtsi}/Atad3a^{tm1a(KOMP)Wtsi}, Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: femur	Weak		2 cont'd_Ig	Cortical bone.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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