Gene Expression Data
Assay Details
Assay
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Reference:
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J:213958
Lang RA, et al., p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis. Development. 2014 Aug;141(16):3177-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7486722
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Gene symbol:
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Shroom3
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Gene name:
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shroom family member 3
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Antibody:
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UPT132
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Detection system:
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Secondary antibody coupled to Alexa Fluor
|
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Results
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Specimen
5G:
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Ctnnd1tm1Abre/Ctnnd1tm1Abre
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: lens placode |
Present |
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5G
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Localized mostly to the zonula adherens and did not appear to strongly colocalize with Ctnnd1 in prominent actomyosin filaments that spanned the apical cortex.
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Specimen
5H:
(close )
Genetic Background:
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involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ
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Mutant Allele(s):
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Ctnnd1tm1Abre/Ctnnd1tm1Abre Tfap2atm1(cre)Moon/Tfap2a+
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Age:
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embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS17: lens pit |
Present |
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5H
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Quantifiably reduced in the apical junctions of hinge point cells, but apical localization in the central portion of the lens pit is unchanged.
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Specimen
5I:
(close )
Genetic Background:
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involves: 129S4/SvJaeSor * 129S6/SvEvTac
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Mutant Allele(s):
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Ctnnd1tm1Abre/Ctnnd1+ Shroom3Gt(ROSA53)Sor/Shroom3+
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Age:
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embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: lens pit |
Present |
|
5I
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Significant reduction in both hinge point and central cells.
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Specimen
S3A:
embryonic day 10.0
(more )
Note: Double labeled: red - Shroom3; green - Ctnnb1 (assay MGI:7486717).
Specimen
S3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Shroom3; green - Ctnnb1 (assay MGI:7486717).
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Specimen
S3C:
embryonic day 10.0
(more )
Note: Double labeled: red - Shroom3; green - Myh10 (assay MGI:7486725).
Specimen
S3C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Shroom3; green - Myh10 (assay MGI:7486725).
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Specimen
S3D:
embryonic day 10.0
(more )
Note: Double labeled: red - Shroom3; green - Ctnnd1 (assay MGI:7486721).
Specimen
S3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Double labeled: red - Shroom3; green - Ctnnd1 (assay MGI:7486721).
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Specimen
S3E/F:
embryonic day 10.5
(more )
Note: Double labeled: red - Shroom3; green - Ctnnd1 (assay MGI:7486721).
Specimen
S3E/F:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Shroom3; green - Ctnnd1 (assay MGI:7486721).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: lens pit |
Present |
|
S3F merge
,
S3F middle
,
S3E middle
,
S3E merge
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Localized mostly to the zonula adherens and did not appear to strongly colocalize with Ctnnd1 in prominent actomyosin filaments that spanned the apical cortex.
|
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