Antibody:	anti-Raldh3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present	Regionally restricted	1A	Expression in subventricular zone of ventromedial lateral ganglionic eminence (LGE).
TS20: telencephalon	Present	Regionally restricted	1A	Expression in sulcus between LGE and medial ganglionic eminence (MGE).
TS20: nasal epithelium	Present		1A
TS20: retina	Present		1A

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Weak	Regionally restricted	1B	Expression domain considerably truncated compared to wild type.
TS20: telencephalon	Weak	Regionally restricted	1B	Low level expression in and around LGE/MGE sulcus.
TS20: nasal epithelium	Present		1B	Expression in nasal epithelium and retina remains at wild-type levels.
TS20: retina	Present		1B	Expression in nasal epithelium and retina remains at wild-type levels.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Present	Regionally restricted	1C	Expression in subventricular zone of lateral ganglionic eminence.
TS24: telencephalon septum	Present		1C

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Weak		1D	Expression domain reduced compared to wild type.
TS24: telencephalon septum	Weak		1D	Expression domain reduced compared to wild type.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Present		3A	Expression domain appears normal compared to wild type.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Weak	Regionally restricted	3B	Expression domain is truncated, similar to that observed in Gsh2 single mutant.