Myh4em1Ktsu
Endonuclease-mediated Allele Detail
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| Symbol: |
Myh4em1Ktsu |
| Name: |
myosin, heavy polypeptide 4, skeletal muscle; endonuclease-mediated mutation 1, Kunihiro Tsuchida |
| MGI ID: |
MGI:7482299 |
| Gene: |
Myh4 Location: Chr11:67128855-67151272 bp, + strand Genetic Position: Chr11, 40.59 cM
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| Alliance: |
Myh4em1Ktsu page
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| Allele Type: |
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Endonuclease-mediated (Null/knockout) |
| Mutation: |
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Insertion
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Mutation details: CRISPR/Cas9 technology using gRNA 5-CACCACAAACACCGATGACTTGG-3 targeting exon 3 (the first coding exon) of both Myh1 and Myh4 genes generated a single nucleotide insertion in Myh4. This targeting event also generated a single nucleotide deletion in Myh1 (Myh1em1Ktsu) in this line.
(J:335578)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Myh4 Mutation: |
97 strains or lines available
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A single targeting event using a gRNA targeting exon 3 introduced simultaneous frameshift mutations into Myh1 and Myh4 generating a strain with both a Myh1 nucleotide deletion ( Myh1em1Ktsu) and a Myh4 nucleotide insertion ( Myh4em1Ktsu) (J:335578). |
| Original: |
J:335578 Hitachi K, et al., Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice. FASEB J. 2023 Jan;37(1):e22692 |
| All: |
1 reference(s) |
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Analysis Tools
