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Gene Expression Data
Assay Details
Assay
Reference: J:335425 Derbyshire ML, et al., Loss of Tbx3 in Mouse Eye Causes Retinal Angiogenesis Defects Reminiscent of Human Disease. Invest Ophthalmol Vis Sci. 2023 May 1;64(5):1
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7482268
Gene symbol: Tbx3
Gene name: T-box 3
Reporter: GFP
Antibody: anti-GFP
Results
Specimen 1H: embryonic day 13.5; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax2 (assay MGI:7482271).
Structure Level Pattern Image Note
TS21: retina Present Graded 1H , 1H'' , 1H'
TS21: optic nerve Weak Single cells 1H' , 1H , 1H'' Few expressing cells in optic nerve head. Low expression in cells co-expressing Pax2.
Specimen 1J: postnatal day 0; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax2 (assay MGI:7482271).
Structure Level Pattern Image Note
TS27: retina Present Regionally restricted 1J Expression in Pax-2 expressing glial progenitors.
Specimen 1J' Dorsal: postnatal day 0; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax2 (assay MGI:7482271).
Structure Level Pattern Image Note
TS27: retina Present Regionally restricted 1J' No difference in expression between dorsal and ventral in Pax2-expressing cells.
Specimen 1J'' Ventral: postnatal day 0; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax2 (assay MGI:7482271).
Structure Level Pattern Image Note
TS27: retina Present Regionally restricted 1J'' No difference in expression between dorsal and ventral in Pax2-expressing cells.
Specimen 1K: postnatal day 2; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Eomes (Tbr2 assay MGI:7482272).
Structure Cell Type Level Pattern Image Note
TS27: retina inner nuclear layer Present 1K (merge) , 1K GFP
TS27: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 1K (merge) , 1K GFP Expression in about half of Tbr2-expressing ganglion cells.
Specimen 1L: postnatal day 7; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax6 (assay MGI:7482273).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 1L (merge) , 1L GFP Expression in Pax6-expressing amacrine cells.
TS28: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 1L GFP , 1L (merge) Expression in Pax6-expressing ganglion cells.
Specimen S2B: embryonic day 13.5; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax2 (assay MGI:7482271).
Structure Level Pattern Image Note
TS21: retina Present Graded S2B , S2B''
Specimen S2D: postnatal day 0; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax2 (assay MGI:7482271).
Structure Level Pattern Image Note
TS27: retina Present Regionally restricted S2D Expression in Pax-2 expressing glial progenitors.
Specimen S2H upper: postnatal day 7; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax6 (assay MGI:7482273).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer amacrine cell Present S2H GFP , S2H Pax6/GFP Expression in Pax6-expressing amacrine cells
TS28: retina ganglion cell layer retinal ganglion cell Present S2H Pax6/GFP , S2H GFP Expression in Pax6-expressing ganglion cells.
Specimen S2H lower: postnatal day 7; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Pou4f2 (Brn3b assay MGI:7482274).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted S2H Brn3b/GFP No co-expression with Pou4f2 (Brn3b).
TS28: retina ganglion cell layer Present S2H Brn3b/GFP
Specimen S2J upper: postnatal day 14; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pax6 (assay MGI:7482273).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer amacrine cell Present S2J Pax6/GFP , S2J' GFP , S2J' Pax6/GFP , S2J GFP Expression in Pax6-expressing amacrine cells.
TS28: retina ganglion cell layer retinal ganglion cell Present S2J Pax6/GFP , S2J' Pax6/GFP , S2J' GFP , S2J GFP Expression in Pax6-expressing ganglion cells.
Specimen S2J lower: postnatal day 14; Tbx3tm2.1Moon/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Sox9 (assay MGI:7482275).
Structure Cell Type Level Pattern Image Note
TS28: retina inner nuclear layer Mueller cell Absent S2J Sox9/GFP , S2J' Sox9/GFP No co-expression with Mueller glia marker Sox9 expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory