Gene Expression Data
Assay Details
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Assay
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Reference:
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J:99320
Wolfe CA, et al., Sex differences in the location of immunochemically defined cell populations in the mouse preoptic area/anterior hypothalamus. Brain Res Dev Brain Res. 2005 Jun 9;157(1):34-41
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7481911
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Gene symbol:
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Esr2
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Gene name:
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estrogen receptor 2 (beta)
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Antibody:
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anti-ERbeta
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1A:
embryonic day 17.5
(more  )
Note: Fixative was 2% acrolein.
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixative was 2% acrolein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
1A
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Expressing cells are ventral and medial.
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| TS25: preoptic area |
Present |
Regionally restricted |
1A
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Expressing cells are ventral and medial.
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Specimen
1B:
embryonic day 17.5
(more )
Note: Fixative was 2% acrolein.
Specimen
1B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixative was 2% acrolein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
1B
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Expressing cells are dorsal and lateral.
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| TS25: preoptic area |
Present |
Regionally restricted |
1B
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Expressing cells are dorsal and lateral.
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Specimen
1C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Nr5a1tm1Klp/Nr5a1tm1Klp
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Agarose
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Note:
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Fixative was 2% acrolein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
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1C
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Expression in this male mutant resembled the female expression.
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| TS25: preoptic area |
Present |
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1C
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Expression in this male mutant resembled the female expression.
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Specimen
2A:
postnatal adult
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hypothalamus |
Present |
Regionally restricted |
2A
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Adult males had a greater area of expressing cells than females.
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| TS28: preoptic area |
Present |
Regionally restricted |
2A
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Adult males had a greater area of expressing cells than females.
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Specimen
2B:
postnatal adult
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Agarose
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Specimen
3A:
postnatal day 0
(more )
Specimen
3A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: hypothalamus |
Present |
Regionally restricted |
3A
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Males were found to have more expressing cells than females.
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| TS27: preoptic area |
Present |
Regionally restricted |
3A
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Males were found to have more expressing cells than females.
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Specimen
3B:
postnatal day 0
(more )
Specimen
3B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Female
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: hypothalamus |
Present |
Regionally restricted |
3B
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Females were found to have less expressing cells than males.
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| TS27: preoptic area |
Present |
Regionally restricted |
3B
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Females were found to have less expressing cells than males.
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6J
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Mutant Allele(s):
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Nr5a1tm1Klp/Nr5a1tm1Klp
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Age:
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postnatal day 0
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Sex:
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Male
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: hypothalamus |
Present |
Regionally restricted |
3C
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Expression in this mutant resembled the female expression.
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| TS27: preoptic area |
Present |
Regionally restricted |
3C
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Expression in this mutant resembled the female expression.
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