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Gene Expression Data
Assay Details
Assay
Reference: J:332245 Liaci C, et al., Loss of ARHGAP15 affects the directional control of migrating interneurons in the embryonic cortex and increases susceptibility to epilepsy. Front Cell Dev Biol. 2022;10:875468
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7470704
Gene symbol: Arhgap15
Gene name: Rho GTPase activating protein 15
Reporter: lacZ
Antibody: anti-beta-Galactosidase (SKU: 085597-CF)
Detection system: Secondary antibody
Results
Specimen 1A E11.5: embryonic day 11.5; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Level Pattern Image Note
TS19: telencephalon Absent 1A E11.5 first , 1A E11.5 second , 1A E11.5 fourth , 1A E11.5 third
Specimen 1A E12.5: embryonic day 12.5; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Level Pattern Image Note
TS20: ganglionic eminence Absent 1A E12.5 first , 1A E12.5 second , 1A E12.5 fourth , 1A E12.5 third
TS20: telencephalon Absent 1A E12.5 first , 1A E12.5 second , 1A E12.5 fourth , 1A E12.5 third
Specimen 1B left: embryonic day 14.5; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Cell Type Level Pattern Image Note
TS22: cerebral cortex marginal layer cortical interneuron Present Regionally restricted 1B''' left , 1B' left , 1B'' left Expression was detected in about 26% of cortical interneurons in the marginal zone.
TS22: cortical plate cortical interneuron Present Regionally restricted 1B' left , 1B''' left , 1B'' left Expression was detected in about 23% of cortical interneurons in the cortical plate.
TS22: cerebral cortex intermediate zone cortical interneuron Present Regionally restricted 1B' left , 1B''' left , 1B'' left Expression was detected in about 18% of cortical interneurons.
TS22: cerebral cortex subventricular zone cortical interneuron Present Regionally restricted 1B' left , 1B''' left , 1B'' left Expression was detected in about 18% of cortical interneurons.
TS22: ganglionic eminence Absent 1B''' left , 1B'' left , 1B' left
Specimen 1B middle: embryonic day 14.5; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Cell Type Level Pattern Image Note
TS22: cerebral cortex marginal layer cortical interneuron Present Regionally restricted 1B'' middle , 1B' middle , 1B''' middle Expression was detected in about 26% of cortical interneurons in the marginal zone.
TS22: cortical plate cortical interneuron Present Regionally restricted 1B''' middle , 1B' middle , 1B'' middle Expression was detected in about 23% of cortical interneurons in the cortical plate.
TS22: cerebral cortex intermediate zone cortical interneuron Present Regionally restricted 1B'' middle , 1B' middle , 1B''' middle Expression was detected in about 18% of cortical interneurons.
TS22: cerebral cortex subventricular zone cortical interneuron Present Regionally restricted 1B'' middle , 1B' middle , 1B''' middle Expression was detected in about 18% of cortical interneurons.
TS22: ganglionic eminence Absent 1B''' middle , 1B'' middle , 1B' middle
Specimen 1B right: embryonic day 14.5; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Cell Type Level Pattern Image Note
TS22: cerebral cortex marginal layer cortical interneuron Present Regionally restricted 1B''' right , 1B'' right , 1B' right Expression was detected in about 26% of cortical interneurons in the marginal zone.
TS22: cortical plate cortical interneuron Present Regionally restricted 1B' right , 1B''' right , 1B'' right Expression was detected in about 23% of cortical interneurons in the cortical plate.
TS22: cerebral cortex intermediate zone cortical interneuron Present Regionally restricted 1B''' right , 1B' right , 1B'' right Expression was detected in about 18% of cortical interneurons.
TS22: cerebral cortex subventricular zone cortical interneuron Present Regionally restricted 1B''' right , 1B' right , 1B'' right Expression was detected in about 18% of cortical interneurons.
TS22: ganglionic eminence Absent 1B' right , 1B''' right , 1B'' right
Specimen 1C P2: postnatal day 2; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex cortical interneuron Present Regionally restricted 1C''' left , 1C'' left , 1C' left Expression was detected in about 35% of cortical interneurons.
Specimen 1C P45: postnatal day 45; Arhgap15tm1Ehi/Arhgap15+, Gad1tm1.1Tama/Gad1+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Gad1 reporter (assay MGI:7470705).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex cortical interneuron Present Regionally restricted 1C'' right , 1C' right , 1C ''' right Expression was detected in about 56% of cortical interneurons.
Specimen 2A PVALB: postnatal day 45; Arhgap15tm1Ehi/Arhgap15+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Pvalb (assay MGI:7470707).
Structure Cell Type Level Pattern Image Note
TS28: somatosensory cortex neuron Present Regionally restricted 2A beta-GAL/PVALB right , 2A beta-GAL/PVALB left , 2A beta-GAL/PVALB middle Expression is in about 57% of Pvalb-positive neurons.
Specimen 2A CALB2: postnatal day 45; Arhgap15tm1Ehi/Arhgap15+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Calb2 (assay MGI:7470708).
Structure Cell Type Level Pattern Image Note
TS28: somatosensory cortex neuron Present 2A beta-GAL/CALB2 right , 2A beta-GAL/CALB2 middle , 2A beta-GAL/CALB2 left Expression is in about 53% of Calb2-positive neurons.
Specimen 2A SST: postnatal day 45; Arhgap15tm1Ehi/Arhgap15+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Sst (assay MGI:7470709).
Structure Cell Type Level Pattern Image Note
TS28: somatosensory cortex neuron Present 2A beta-GAL/SST right , 2A beta-GAL/SST left , 2A beta-GAL/SST middle Expression is in about 53% of Sst-positive neurons.
Specimen 2A VIP: postnatal day 45; Arhgap15tm1Ehi/Arhgap15+ (more )
Note: Double labeled: red - Arhgap15 reporter; green - Vip (assay MGI:7470710).
Structure Cell Type Level Pattern Image Note
TS28: somatosensory cortex neuron Present 2A beta-GAL/VIP right , 2A beta-GAL/VIP left , 2A beta-GAL/VIP middle Expression is in about 52% of Vip-positive neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory