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Gene Expression Data
Assay Details
Assay
Reference: J:309709 Trujillo CA, et al., Kinin-B2 receptor activity determines the differentiation fate of neural stem cells. J Biol Chem. 2012 Dec 28;287(53):44046-61
Assay type: RT-PCR
MGI Accession ID: MGI:7469876
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Probe: Beta3-Tubulin-pF, Beta3-Tubulin-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
Image: 10E ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 111 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
Adult WT P adult TS28: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Not Specified
Adult B2BkR-/- P adult TS28: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Bdkrb2tm1Jfh/Bdkrb2tm1JfhNot Specified
E9.5 WT E9.5 TS15: future brain Ambiguous 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Not Specified
E9.5 B2BkR-/- E9.5 TS15: future brain Ambiguous 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Bdkrb2tm1Jfh/Bdkrb2tm1JfhNot Specified
E11.5 WT E11.5 TS19: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Not Specified
E11.5 B2BkR-/- E11.5 TS19: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Bdkrb2tm1Jfh/Bdkrb2tm1JfhNot Specified
E14.5 WT E14.5 TS22: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Not Specified
E14.5 B2BkR-/- E14.5 TS22: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Bdkrb2tm1Jfh/Bdkrb2tm1JfhNot Specified
E17.5 WT E17.5 TS25: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Not Specified
E17.5 B2BkR-/- E17.5 TS25: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Bdkrb2tm1Jfh/Bdkrb2tm1JfhNot Specified
P0 WT P0 TS27: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Not Specified
P0 B2BkR-/- P0 TS27: brain Present 3 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Bdkrb2tm1Jfh/Bdkrb2tm1JfhNot Specified
Notes:
(a) The mutant showed significantly less expression after E11.5 compared with the WT littermate.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory