Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu
Targeted Allele Detail
|
|
| Symbol: |
Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu |
| Name: |
intergenic site 2; targeted mutation 1, Ming Xu |
| MGI ID: |
MGI:7469624 |
| Synonyms: |
p21-Cre |
| Gene: |
Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
|
| Alliance: |
Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:335288
|
| Parent Cell Line: |
Not Specified (ES Cell)
|
| Strain of Origin: |
Not Specified
|
|
| Allele Type: |
|
Targeted (Inducible, Recombinase, Reporter) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Cdkn1a (MGI:104556) |
|
|
| |
|
Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert a 3,225-bp mouse Cdkn1a (p21) promoter fragment followed by a bicistronic message consisting of the coding sequence for Cre fused to an ERT2 domain, an IRES followed by an ORF coding for enhanced GFP into the intergenic site 2 locus (Hipp11). This construct was microinjected into the pronucleus of recipient zygotes containing attP sites in the Hipp11 locus. Site-specific recombination occurred between the attB sites in the plasmid and attP sites in the H11 genomic locus. Positive embryonic stem cell clones were then implanted into C57BL/6 females.
(J:335288)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Cdkn1a
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Igs2 Mutation: |
69 strains or lines available
|
|
| Original: |
J:335288 Wang B, et al., An inducible p21-Cre mouse model to monitor and manipulate p21-highly-expressing senescent cells in vivo. Nat Aging. 2021 Oct;1(10):962-973 |
| All: |
3 reference(s) |
|
Analysis Tools
