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Gene Expression Data
Assay Details
Assay
Reference: J:335071 Siddam AD, et al., High-Throughput Transcriptomics of Celf1 Conditional Knockout Lens Identifies Downstream Networks Linked to Cataract Pathology. Cells. 2023 Apr 1;12(7)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7467440
Gene symbol: Cryg
Gene name: crystallin gamma complex
Antibody: anti-Gamma crystallin P-18 (sc-22415)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S1A Control: embryonic day 16.5; Celf1tm1Ics/Celf1tm1Ics (more )
Note: Double labeled: green - Cryg; red - E-cad (Cdh1 assay MGI:7467439).
Structure Level Pattern Image Note
TS24: lens fiber Present S1A Control
Specimen S1A Celf1 cKO#1: embryonic day 16.5; Celf1tm1Cba/Celf1tm1Ics, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant. Double labeled: green - Cryg; red - E-cad (Cdh1 assay MGI:7467439).
Structure Level Pattern Image Note
TS24: lens fiber Weak S1A Celf1 cKO#1 Expression reduced in fiber cells.
Specimen S1B Control: embryonic day 16.5; Celf1tm1Ics/Celf1tm1Ics (more )
Structure Level Pattern Image Note
TS24: lens fiber Present S1B Control , S1B' Control
Specimen S1B Celf1 cKO#2: embryonic day 16.5; Celf1tm1Cba/Celf1tm1Ics, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: lens fiber Weak S1B' Celf1 cKO#2 , S1B Celf1 cKO#2 Expression reduced in fiber cells.
Specimen S1B Celf1 cKO#3: embryonic day 16.5; Celf1tm1Cba/Celf1tm1Ics, Tg(Pax6-cre,GFP)1Pgr/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS24: lens fiber Weak S1B Celf1 cKO#3 , S1B' Celf1 cKO#3 Expression reduced in fiber cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory