About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:164280 Bowles J, et al., FGF9 suppresses meiosis and promotes male germ cell fate in mice. Dev Cell. 2010 Sep 14;19(3):440-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466386
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phosphoH2AX (#05-636; JBW301)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1D XY Wt - upper: embryonic day 15.5 (more )
Note: Double labeled: red - H2ax; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of testis Absent 1D XY WT upper In XY gonads germ cells are not in an advanced stage of meiosis.
Specimen 1D XY Cyp26b1 KO - upper: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - H2ax; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present 1D XY Cyp26b1 KO upper Unlike in wild type XY gonads, cells are in an advanced stage of meiosis.
Specimen 1D XX Wt - upper: embryonic day 15.5 (more )
Note: Double labeled: red - H2ax; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of ovary Present 1D XX WT upper In XX gonads germ cells are in an advanced stage of meiosis.
Specimen S4B - left: embryonic day 15.5; Fgf9tm1Dor/Fgf9tm1Dor (more )
Note: Double labeled: red - H2ax; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present S4B left Unlike in wild type XY gonads, cells are in an advanced stage of meiosis.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory