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Gene Expression Data
Assay Details
Assay
Reference: J:164280 Bowles J, et al., FGF9 suppresses meiosis and promotes male germ cell fate in mice. Dev Cell. 2010 Sep 14;19(3):440-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466384
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1D XY Wt - upper: embryonic day 15.5 (more )
Note: Double labeled: green - Ddx4; red - H2ax (assay MGI:7466386).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1D XY WT upper
Specimen 1D XY Cyp26b1 KO - upper: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: green - Ddx4; red - H2ax (assay MGI:7466386).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present 1D XY Cyp26b1 KO upper
Specimen 1D XX Wt - upper: embryonic day 15.5 (more )
Note: Double labeled: green - Ddx4; red - H2ax (assay MGI:7466386).
Structure Level Pattern Image Note
TS23: germ cell of ovary Present 1D XX WT upper
Specimen 1D XY Wt - lower: embryonic day 15.5 (more )
Note: Double labeled: green - Ddx4; red - Pou5f1 (assay MGI:7466387).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1D XY WT lower
Specimen 1D XY Cyp26b1 KO - lower: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: green - Ddx4; red - Pou5f1 (assay MGI:7466387).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present 1D XY Cyp26b1 KO lower
Specimen 1D XX Wt - lower: embryonic day 15.5 (more )
Note: Double labeled: green - Ddx4; red - Pou5f1 (assay MGI:7466387).
Structure Level Pattern Image Note
TS23: germ cell of ovary Present 1D XX WT lower
Specimen S4B - left: embryonic day 15.5; Fgf9tm1Dor/Fgf9tm1Dor (more )
Note: Double labeled: green - Ddx4; red - H2ax (assay MGI:7466386).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present S4B left
Specimen S4B - right: embryonic day 15.5; Fgf9tm1Dor/Fgf9tm1Dor (more )
Note: Double labeled: green - Ddx4; red - Pou5f1 (assay MGI:7466387).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present S4B right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory