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Gene Expression Data
Assay Details
Assay
Reference: J:164280 Bowles J, et al., FGF9 suppresses meiosis and promotes male germ cell fate in mice. Dev Cell. 2010 Sep 14;19(3):440-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466387
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4 (C-10; sc-5279)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1D XY Wt - lower: embryonic day 15.5 (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1D XY WT lower
Specimen 1D XY Cyp26b1 KO - lower: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present 1D XY Cyp26b1 KO lower As in wild type XY gonads, expression is retained in the germ cells.
Specimen 1D XX Wt - lower: embryonic day 15.5 (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of ovary Trace 1D XX WT lower
Specimen S1C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Pou5f1; green - Sycp3 (assay MGI:7466382).
Structure Level Pattern Image Note
TS23: germ cell of gonad Present S1C merge , S1C OCT4 Unlike in wild type XY gonads, the germ cells express a pluripotency marker (Pou5f1) and a marker for advanced meiosis (Sycp3).
Specimen S4B - left: embryonic day 15.5; Fgf9tm1Dor/Fgf9tm1Dor (more )
Note: Double labeled: red - Pou5f1; green - Ddx4 (assay MGI:7466384).
Structure Level Pattern Image Note
TS23: germ cell of gonad Absent S4B right Unlike in wild type XY gonads, expression is not seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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