Gene Expression Data
Assay Details
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Assay
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Reference:
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J:164280
Bowles J, et al., FGF9 suppresses meiosis and promotes male germ cell fate in mice. Dev Cell. 2010 Sep 14;19(3):440-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7466382
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Gene symbol:
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Sycp3
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Gene name:
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synaptonemal complex protein 3
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Results
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Specimen
1D XY Wt - center:
embryonic day 15.5
(more  )
Specimen
1D XY Wt - center:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of testis |
Absent |
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1D XY WT center
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In XY gonads germ cells are not in an advanced stage of meiosis.
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Specimen
1D XY Cyp26b1 KO - center:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Cyp26b1tm1Hmd/Cyp26b1tm1Hmd
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of gonad |
Present |
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1D XY Cyp26b1 KO center
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Unlike in wild type XY gonads, cells are in an advanced stage of meiosis.
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Specimen
1D XX Wt - center:
embryonic day 15.5
(more )
Specimen
1D XX Wt - center:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 15.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of ovary |
Present |
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1D XX WT center
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In XX gonads germ cells are in an advanced stage of meiosis.
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Specimen
S1C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Cyp26b1tm1Hmd/Cyp26b1tm1Hmd
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Sycp3; red - Pou5f1 (assay MGI:7466387).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of gonad |
Present |
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S1C SYCP3
,
S1C merge
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Unlike in wild type XY gonads, the germ cells express a pluripotency marker (Pou5f1) and a marker for advanced meiosis (Sycp3).
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Specimen
S4B - center:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Fgf9tm1Dor/Fgf9tm1Dor
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of gonad |
Present |
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S4B center
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Unlike in wild type XY gonads, cells are in an advanced stage of meiosis.
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Analysis Tools