About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:184591 Aoyama M, et al., Spatial Restriction of Bone Morphogenetic Protein Signaling in Mouse Gastrula through the mVam2-Dependent Endocytic Pathway. Dev Cell. 2012 Jun 12;22(6):1163-75
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7464737
Gene symbol: Rab7
Gene name: RAB7, member RAS oncogene family
Antibody: anti-rab7
Detection system: Secondary antibody
Assay notes: Two Rab7 antibodies were described, but the one used in this assay was not detailed. Anti-rab7 MGI:7464562 may have been used in this assay.
Results
Specimen 1C WT sagittal: embryonic day 6.5 (more )
Note: Double labeled: green - Rab7; red - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Punctate 1C WT sagittal Punctate expression localized to apical vacuoles in visceral endoderm.
Specimen 1C WT parasagittal: embryonic day 6.5 (more )
Note: Double labeled: green - Rab7; red - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Punctate 1C WT parasagittal Punctate expression localized to apical vacuoles in visceral endoderm.
Specimen 1C mVam2-/- sagittal: embryonic day 6.5; Vps41tm1.1Ywd/Vps41tm1.1Ywd (more )
Note: Double labeled: green - Rab7; red - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Regionally restricted 1C mVam2-/- sagittal Expression frequently decorated entire boundary of spherical structures.
Specimen 1C mVam2-/- parasagittal: embryonic day 6.5; Vps41tm1.1Ywd/Vps41tm1.1Ywd (more )
Note: Double labeled: green - Rab7; red - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Regionally restricted 1C mVam2-/- parasagittal Expression frequently decorated entire boundary of spherical structures.
Specimen 6A rab7 WT: embryonic day 6.2 (more )
Note: Triple labeled: green - Rab7 red - Bmpr1a (assay MGI:7464760); blue - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 6A WT inset , 6A WT
Specimen 6A rab7 mVam2-/-: embryonic day 6.2; Vps41tm1.1Ywd/Vps41tm1.1Ywd (more )
Note: Triple labeled: green - Rab7 red - Bmpr1a (assay MGI:7464760); blue - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS8: primitive endoderm Present 6A mVam2-/- inset , 6A mVam2-/-
Specimen S5A rab7 wild-type: embryonic day 6.0 (more )
Note: Triple labeled: green - Rab7 red - Bmpr1a (assay MGI:7464760); blue - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS8: primitive endoderm Present S5A Bmpr1a/lamp2/rab7 wild-type
Specimen S5A rab7 mVam2-/-: embryonic day 6.0; Vps41tm1.1Ywd/Vps41tm1.1Ywd (more )
Note: Triple labeled: green - Rab7 red - Bmpr1a (assay MGI:7464760); blue - Lamp2 (assay MGI:7464741).
Structure Level Pattern Image Note
TS8: primitive endoderm Present S5A Bmpr1a/lamp2/rab7 mVam2-/-
Specimen S5C rab7 wild-type: embryonic day 6.0 (more )
Note: Double labeled: green - Rab7; red - Bmpr1a (assay MGI:7464760).
Structure Level Pattern Image Note
TS8: primitive endoderm Present Regionally restricted S5C wild-type (lower) , S5C wild-type (upper) Expression in visceral endoderm overlying extraembryonic ectoderm. Expression in apical vacuoles.
Specimen S5C rab7 mVam2-/-: embryonic day 6.0; Vps41tm1.1Ywd/Vps41tm1.1Ywd (more )
Note: Double labeled: green - Rab7; red - Bmpr1a (assay MGI:7464760).
Structure Level Pattern Image Note
TS8: primitive endoderm Present Regionally restricted S5C mVam2-//- (upper) , S5C mVam2-/- (lower) Expression in visceral endoderm overlying extraembryonic ectoderm. Expression in apical vacuoles.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory