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Gene Expression Data
Assay Details
Assay
Reference: J:314694 Douchi D, et al., Induction of Gastric Cancer by Successive Oncogenic Activation in the Corpus. Gastroenterology. 2021 Dec;161(6):1907-1923.e26
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7461884
Gene symbol: Pgc
Gene name: progastricsin (pepsinogen C)
Reporter: mCherry
Antibody: anti-RFP
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1B - lesser: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter; blue - Pgc protein (assay MGI:7463981); green - Cdh1 (assay MGI:7463981).
Structure Level Pattern Image Note
TS28: stomach lesser curvature Present Regionally restricted 1B left upper , 1B right upper Expression is seen in 56.9% of the corpus units in the lesser curvature.
Specimen 1B - greater: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter; blue - Pgc protein (assay MGI:7463981); green - Cdh1 (assay MGI:7463981).
Structure Level Pattern Image Note
TS28: stomach greater curvature Present Regionally restricted 1B right lower , 1B left lower Expression is seen in 60.7% of the corpus units in the lesser curvature.
Specimen 1F: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter; blue - Pgc protein (assay MGI:7463981); green - Mki67 (assay MGI:7463939).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present Non-Uniform 1F Merge , 1F upper inset , 1F Ki67 mCherry , 1F lower inset Nearly all expressing cells are found in the corpus base; only a few are found in the neck and isthmus.
TS28: stomach glandular region epithelium peptic cell Present 1F Merge , 1F Ki67 mCherry Expression is seen in chief cells.
Specimen 1H - H,K-ATPase: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Double labeled: red - Pgc; green - Atp4a (assay MGI:7463974).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present 1H left , 1H left red inset , 1H left merge inset
TS28: stomach glandular region epithelium parietal cell Absent 1H left merge inset , 1H left , 1H left red inset There is no expression in parietal cells.
Specimen 1H - ChgA: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Double labeled: red - Pgc; green - Chga (assay MGI:7463977).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present 1H center , 1H center merge inset , 1H center red inset
TS28: stomach glandular region epithelium enteroendocrine cell Absent 1H center red inset , 1H center merge inset , 1H center There is no expression in enteroendocrine cells.
Specimen 1H - Muc5ac: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Double labeled: red - Pgc; green - Muc5ac (assay MGI:7463978).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present 1H right
TS28: stomach glandular region epithelium foveolar cell of stomach Absent 1H right There is no expression in pit cells
Specimen S2G: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter (mCherry); green - Pgc reporter (Cre; assay MGI:7461913); blue - Mki67 (assay MGI:7463939).
Structure Level Pattern Image Note
TS28: stomach glandular region gastric gland Present Non-Uniform S2G left , S2G center There is expression in the gland base of the corpus. Cre was expressed in most mCherry-expressing cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory