About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:333400 George RM, et al., Single cell evaluation of endocardial Hand2 gene regulatory networks reveals HAND2-dependent pathways that impact cardiac morphogenesis. Development. 2023 Feb 15;150(3):dev201341
Assay type: RNA in situ
MGI Accession ID: MGI:7461705
Gene symbol: Klf2
Gene name: Kruppel-like transcription factor 2 (lung)
Probe: Klf2 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2B: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Hand2tm1.1Majh/Hand2+ (more )
Structure Level Pattern Image Note
TS20: ventricle endocardial lining Present Regionally restricted 2B' , 2B Robust expression was detected.
TS20: atrioventricular canal endocardial lining Present 2B , 2B' Expression was particularly within areas of high shear stress.
TS20: vasculature Present 2B
Specimen 2D: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Hand2tm1.1Majh/Hand2tm1.1Majh, Nfatc1tm1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: ventricle endocardial lining Weak Regionally restricted 2D' , 2D Expression was significantly reduced in the trabecular endocardium of the ventricles in the H2CKO hearts compared with controls.
TS20: atrioventricular canal endocardial lining Present 2D' , 2D Expression in the AV canal endocardial lining was maintained in the H2CKO hearts.
TS20: vasculature Present 2D Staining in systemic vasculature was maintained in the mutant.
Specimen 5A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: atrioventricular canal endocardial lining Present 5A
TS19: ventricle endocardial lining Present 5A
Specimen 5B: embryonic day 11.5; Rr417em1Abfi/Rr417em1Abfi (more )
Structure Level Pattern Image Note
TS19: atrioventricular canal endocardial lining Weak 5B A visible decrease of expression was observed in the mutant.
TS19: ventricle endocardial lining Weak 5B A visible decrease of expression was observed in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory