Gene Expression Data
Assay Details
Assay
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Reference:
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J:334332
Pilaz LJ, et al., Subcellular mRNA localization and local translation of Arhgap11a in radial glial progenitors regulates cortical development. Neuron. 2023 Mar 15;111(6):839-856.e5
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7461494
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Gene symbol:
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Arhgap11a
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Gene name:
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Rho GTPase activating protein 11A
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Antibody:
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anti-ARHGAP11A (A303-097A)
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Assay notes:
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Two Arhgap11a antibodies were described, but the one used in this assay was not detailed. Anti-Arhgap11a (ab113261) MGI:7461491 may have been used in this assay.
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Results
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Specimen
1E:
embryonic day 11.5
(more )
Specimen
1E:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1F:
embryonic day 13.5
(more )
Specimen
1F:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex marginal layer |
forebrain radial glial cell |
Present |
Regionally restricted |
1F ARHGAP11A
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Expression enriched near pial surface at presumptive radial glial cell endfeet and along basal processes.
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Specimen
1G:
embryonic day 15.5
(more )
Specimen
1G:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
1G ARHGAP11A
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Localization near pial surface especially visible at later stages.
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Specimen
1H ARHGAP11A:
embryonic day 15.5
(more )
Note: Double labeled: red - Arhgap11a; green - Nes (assay MGI:7461495).
Specimen
1H ARHGAP11A:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Arhgap11a; green - Nes (assay MGI:7461495).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: cerebral cortex marginal layer |
forebrain radial glial cell |
Present |
Regionally restricted |
1H Merge
,
1H ARHGAP11A
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Expression overlapping with Nestin expression in basal process and endfeet at pial surface.
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Specimen
1M ARHGAP11A IF:
embryonic day 16.5
(more )
Note: Double labeled: green - Arhgap11a protein; red - Arhgap11a RNA (assay MGI:7461498).
Specimen
1M ARHGAP11A IF:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Arhgap11a protein; red - Arhgap11a RNA (assay MGI:7461498).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex marginal layer |
forebrain radial glial cell |
Present |
Regionally restricted |
1M inset
,
1M
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Expression in radial glial cell endfeet at pia, colocalizing with Arhgap11a mRNA expression.
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