Probe:	Barhl2 cDNA
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1D	Expressed throughout much of the diencephalon, with strongest expression in alar regions overlapping the middle of the diencephalic domain of Pax6 expression (summarized in Fig 1M).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1E	Expressed throughout much of the diencephalon, with strongest expression in alar regions overlapping the middle of the diencephalic domain of Pax6 expression (summarized in Fig 1M).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1F	Expressed throughout much of the diencephalon, with strongest expression in alar regions overlapping the middle of the diencephalic domain of Pax6 expression. No expression in the telencephalon (summarized in Fig 1M).

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1J	Expressed in the basal plate (summarized in Fig 1N).

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1K	The expression domain had consolidated into a band of neuroepithelium running from ventral to dorsal, flanked by regions of diencephalic neuroepithelium with little or no expression (summarized in Fig. 1N).
TS15: telencephalon	Absent		1K

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	1L	The expression domain had consolidated into a band of neuroepithelium running from ventral to dorsal, flanked by regions of diencephalic neuroepithelium with little or no expression (summarized in Fig. 1N).
TS15: telencephalon	Absent		1L

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2D	Expressed in the zona limitans intrathalamica (ZLI) and throughout the majority of the thalamic ventricular zone, but not in the prethalamus and a narrow strip of progenitor cells immediately caudal to the ZLI.
TS17: forebrain-midbrain boundary region	Present	Regionally restricted	2D	Expressed in the pretectum.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2E	Expressed throughout the majority of the thalamic ventricular zone, but not in the prethalamus.
TS17: forebrain-midbrain boundary region	Present	Regionally restricted	2E	Expressed in the pretectum.
TS17: eye	Absent		2E
TS17: telencephalon	Absent		2E

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2F	Expressed throughout the majority of the thalamic ventricular zone, but not in the prethalamus.
TS17: eye	Absent		2F
TS17: telencephalon	Absent		2F
TS17: forebrain	Present	Regionally restricted	2F	Expressed within the mantle zone of the developing eminentia thalami.

Structure	Level	Pattern	Image	Note
TS19: forebrain-midbrain boundary region	Present	Regionally restricted	2J	Expressed in the pretectum.
TS19: diencephalon	Present	Regionally restricted	2J	Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone and the hypothalamus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2K	Expressed in zona limitans intrathalamica (strongly), the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone and the hypothalamus. Expression remained low or absent in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS19: prethalamus	Absent		2K

Structure	Level	Pattern	Image	Note
TS19: telencephalon mantle layer	Present	Regionally restricted	2L	Expressed in differentiating cells in the mantle zone of the ventral telencephalon, in regions where Pax6 had no expression.
TS19: diencephalon	Present	Regionally restricted	2L	Expressed in the hypothalamus, which was complementary to the expression of Pax6.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3D	Expressed in the progenitor zone. Still expressed in a gradient, with levels increasing from caudal to rostral sections, but its levels were as high or higher than those in other forebrain regions.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	3D	Expressed in the pretectum.
TS20: hypothalamus	Present		3D

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3E	Expressed in the progenitor zone. Still expressed in a gradient, with levels increasing from caudal to rostral sections, but its levels were as high or higher than those in other forebrain regions.
TS20: zona limitans intrathalamica	Present		3E

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	3F	Expressed in the postmitotic zone of the eminentia thalami (Pax6-).
TS20: thalamus	Present	Regionally restricted	3F	Expressed in the progenitor zone. Still expressed in a gradient, with levels increasing from caudal to rostral sections, but its levels were as high or higher than those in other forebrain regions.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	3J	Expressed in the progenitor zone. Still expressed in a gradient but its levels were as high or higher than those in other forebrain regions.
TS21: zona limitans intrathalamica	Present		3J

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present		3K
TS21: forebrain	Present	Regionally restricted	3K	Expressed in the eminentia thalami (Pax6-).

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	3L	Expressed in the postmitotic zone of the eminentia thalami (Pax6-).
TS21: thalamus	Present		3L

Structure	Level	Pattern	Image	Note
TS19: diencephalon ventricular layer	Present	Regionally restricted	9A	Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: diencephalon ventricular layer	Present	Regionally restricted	9B	Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone and zona limitans intrathalamica. No in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS19: prethalamus	Absent		9B

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	9C	Expressed in the ventro-laterally positioned cells.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		9D	Expression spanned all or most of the mediolateral width of the thalamic neuroepithelium, most likely due to the absence of normal mantle zone development. Expressed within the expanded zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	9E	Expression spanned all or most of the mediolateral width of the thalamic neuroepithelium, most likely due to the absence of normal mantle zone development. Expressed within the expanded zona limitans intrathalamica. Weaker expression in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS19: prethalamus	Absent		9E

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	9F	Expressed in the ventro-laterally positioned cells, similar as in the wild-type.

Structure	Level	Pattern	Image	Note
TS20: thalamus ventricular layer	Present		9G	Expressed in the region of progenitor cells (pTh-C domain).

Structure	Level	Pattern	Image	Note
TS20: thalamus ventricular layer	Present		9H	Expressed in the region of progenitor cells (pTh-C domain). No expression in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS20: prethalamus	Absent		9H

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	9I	Expressed in the ventro-laterally positioned cells.
TS20: prethalamus	Absent		9I

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		9J	Expression spanned all or most of the mediolateral width of the thalamic neuroepithelium, most likely due to the absence of normal mantle zone development. Elevated expression was seen.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	9J	Elevated expression was seen in the pretectum.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		9K	Expression spanned all or most of the mediolateral width of the thalamic neuroepithelium, most likely due to the absence of normal mantle zone development. Elevated expression was seen. Weaker expression in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS20: zona limitans intrathalamica	Present		9K	Expressed within the expanded zona limitans intrathalamica.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	9K	Elevated expression was seen in the pretectum.
TS20: prethalamus	Absent		9K

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present		9L	Expressed within the expanded zona limitans intrathalamica.
TS20: telencephalon	Present	Regionally restricted	9L	Expressed in the ventro-laterally positioned cells, similar as in the wild-type.