Probe:	Pax6 cDNA16
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1A	Expressed throughout most of the alar diencephalon, but was absent from the basal plate (summarized in Fig 1M).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1B	Expressed throughout most of the alar diencephalon and telencephalon, but was absent from their basal plate (summarized in Fig 1M).

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	1C	Expressed throughout most of the alar diencephalon and telencephalon, but was absent from their basal plate (summarized in Fig 1M).

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	1G	Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	1H	Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
TS15: telencephalon	Strong	Regionally restricted	1H	Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Strong	Regionally restricted	1I	Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
TS15: telencephalon	Strong	Regionally restricted	1I	Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong	Regionally restricted	2A	Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI). In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
TS17: telencephalon	Strong		2A
TS17: forebrain-midbrain boundary region	Strong	Regionally restricted	2A	Expressed in the pretectum.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong	Regionally restricted	2B	Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI). In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
TS17: retina	Strong		2B
TS17: telencephalon	Strong		2B
TS17: forebrain-midbrain boundary region	Strong	Regionally restricted	2B	Expressed in the pretectum.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong	Regionally restricted	2C	Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI) and the mantle cells of the developing eminentia thalami. In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
TS17: telencephalon	Strong		2C

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present		2G	Many telencephalic progenitor cells express Pax6, they did not express Barhl2.
TS19: diencephalon	Present	Regionally restricted	2G	Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: prethalamus	Strong		2H
TS19: diencephalon	Present	Regionally restricted	2H	Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone. Expression remained low or absent in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica. No expression in zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS19: telencephalon mantle layer	Present	Regionally restricted	2I	Differentiating cells in the mantle zone of the ventral telencephalon in certain regions had no expression.
TS19: diencephalon	Present	Regionally restricted	2I	Expressed in the hypothalamus, which was complementary to the expression of Barhl2.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		3A	Expressed in both progenitor and postmitotic zones.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	3A	Expressed in the pretectum.
TS20: telencephalon	Present		3A

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present	Graded	3B	Expressed in both progenitor and postmitotic zones. Still expressed in a gradient but its levels are relatively low compared to those in other forebrain regions.
TS20: pallium	Present	Regionally restricted	3B	Expressed in the progenitor zone.
TS20: thalamus	Present	Regionally restricted	3B	Expressed in the progenitor zone.
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	3B	Expressed in the pretectum.
TS20: telencephalon	Present		3B

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		3C	Expressed in both progenitor and postmitotic zones.
TS20: pallium	Present	Regionally restricted	3C	Expressed in the progenitor zone.
TS20: thalamus	Present	Regionally restricted	3C	Expressed in the progenitor zone.

Structure	Level	Pattern	Image	Note
TS21: prethalamus	Present		3G	Expressed in both progenitor and postmitotic zones.

Structure	Level	Pattern	Image	Note
TS21: prethalamus	Present		3H	Expressed in both progenitor and postmitotic zones.

Structure	Level	Pattern	Image	Note
TS21: prethalamus	Present		3I