Probe:	Nwd2 cDNA2
Probe preparation:	Antisense labelled with alkaline phosphatase RNA
Visualized with:	Fluorescence

Structure	Cell Type	Level	Pattern	Image	Note
TS22: habenula	neuron	Present		5C , 5E , 5B , 5F , 5F'	Co-labeling was detected in all Elavl3/4-immunopositive cells in the embryonic habenula. Almost all Nwd2-positive cells were positive for Elavl3/4.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	neuron	Present		5H , 5L , 5K , 5L' , 5I	Expression was detected in the medial habenula. Co-labeling with Elavl3/4 was detected.
TS28: lateral habenular nucleus	neuron	Present	Scattered	5L , 5H , 5L'' , 5I , 5K	Expression was observed in a few scattered neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	neuron	Present		5N , 5O , 5R' , 5Q , 5R	Expression was detected in the medial habenula. Co-labeling with Elavl3/4 was detected.
TS28: lateral habenular nucleus	neuron	Present	Scattered	5R'' , 5N , 5Q , 5O , 5R	Expression was observed in a few scattered neurons. No Nwd2-positive;Elavl3/4-negative cells were seen in lateral habenula.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: habenula	neuron	Present		6C' , 6B , 6C	Nwd2 was expressed in the habenula earlier than Rbfox3 labeling was detected.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	neuron	Present		6F' , 6E , 6F	All Nwd2-positive neurons were also positive for Rbfox3.
TS28: lateral habenular nucleus	neuron	Present		6E , 6F'' , 6F	All Nwd2-positive neurons were also positive for Rbfox3.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	neuron	Present		6I' , 6I , 6H	All Nwd2-positive neurons were also positive for Rbfox3.
TS28: lateral habenular nucleus	neuron	Present		6I , 6H , 6I''	All Nwd2-positive neurons were also positive for Rbfox3.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: habenula	neuron	Present		6K , 6L' , 6L	Nwd2 was expressed in the habenula earlier than Chat labeling was detected.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	cholinergic neuron	Present	Regionally restricted	6N , 6O' , 6O	Expression in the ventral medial habenula overlapped with Chat expression in cholinergic neurons.
TS28: medial habenular nucleus	neuron	Present	Regionally restricted	6O , 6O'' , 6N	Expression of Nwd2 in dorsal medial habenula was devoid of Chat expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: medial habenular nucleus	cholinergic neuron	Present	Regionally restricted	6Q , 6R' , 6R	Expression in the ventral medial habenula overlapped with Chat expression in cholinergic neurons.
TS28: medial habenular nucleus	neuron	Present	Regionally restricted	6R , 6Q , 6R''	Expression of Nwd2 in dorsal medial habenula was devoid of Chat expression.