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Gene Expression Data
Assay Details
Assay
Reference: J:225747 Jayewickreme CD, et al., Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol. 2015 Sep 1;405(1):21-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7448913
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki67
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 5A: embryonic day 17.5 (more )
Note: Double labeled: red - Mki67; green - Acta2 (assay MGI:7448911).
Structure Cell Type Level Pattern Image Note
TS25: stomach wall smooth muscle cell Present 5A middle , 5A lower

Specimen 5B: embryonic day 17.5 (more )
Note: Double labeled: red - Mki67; green - Acta2 (assay MGI:7448911).
Structure Cell Type Level Pattern Image Note
TS25: intestine smooth muscle enteric smooth muscle cell Present 5B middle , 5B lower

Specimen 5C: embryonic day 17.5; Gt(ROSA)26Sortm1(Barx1)Shiv/Gt(ROSA)26Sor+, Nkx3-2tm1(cre)Wez/Nkx3-2+ (more )
Note: Conditional mutant. Double labeled: red - Mki67; green - Acta2 (assay MGI:7448911).
Structure Cell Type Level Pattern Image Note
TS25: intestine smooth muscle enteric smooth muscle cell Present 5C middle , 5C lower Increased proliferation of muscle, and reduced proliferation of non-muscle, cells compared to wild-type littermates (summarized in 5F).

Specimen 5D: embryonic day 17.5; Barx1tm1Shiv/Barx1tm1Shiv (more )
Note: Double labeled: red - Mki67; green - Acta2 (assay MGI:7448911).
Structure Cell Type Level Pattern Image Note
TS25: stomach wall smooth muscle cell Present 5D middle , 5D lower Reduced proliferation of muscle, and increased proliferation of non-muscle, cells compared to wild-type littermates (summarized in 5F).


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory