Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:225747 Jayewickreme CD, et al., Control of stomach smooth muscle development and intestinal rotation by transcription factor BARX1. Dev Biol. 2015 Sep 1;405(1):21-32
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7448911
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-SMA-A
Detection system:	Secondary antibody coupled to fluorescein

Results

5A lower ©

5A middle ©

5A upper ©

Specimen 5A: embryonic day 17.5 (more

)
Note: Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: stomach wall	smooth muscle cell	Present		5A upper , 5A lower , 5A middle

5B lower ©

5B middle ©

5B upper ©

Specimen 5B: embryonic day 17.5 (more

)
Note: Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: intestine smooth muscle	enteric smooth muscle cell	Present		5B middle , 5B upper , 5B lower

5C lower ©

5C middle ©

5C upper ©

Specimen 5C: embryonic day 17.5; Gt(ROSA)26Sor^{tm1(Barx1)Shiv}/Gt(ROSA)26Sor⁺, Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: intestine smooth muscle	enteric smooth muscle cell	Present		5C middle , 5C lower , 5C upper	Significant smooth muscle expansion was seen (summarized in 5E).

5D lower ©

5D middle ©

5D upper ©

Specimen 5D: embryonic day 17.5; Barx1^tm1Shiv/Barx1^tm1Shiv (more

)
Note: Double labeled: green - Acta2; red - Mki67 (assay MGI:7448913).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: stomach wall	smooth muscle cell	Present		5D middle , 5D lower , 5D upper	Significant smooth muscle depletion was seen (summarized in 5E).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/21/2024 MGI 6.23