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Gene Expression Data
Assay Details
Assay
Reference: J:278612 Miltner AM, et al., A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci. 2019 Jun 14;20(12):2903
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7444411
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system: Alexa Fluor
Results
Specimen 2D': postnatal adult; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: teal - Tuj1 (Tubb3); red - Brn3b (Pou4f2) reporter (assay MGI:7444407).
Structure Cell Type Level Pattern Image Note
TS28: retina retinal ganglion cell Present Regionally restricted 2D' Expression in retinal ganglion cells. Co-localization with Brn3b (Pou4f2) reporter expression in axons.

Specimen 4C': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: green - Tuj1 (Tubb3); red - Brn3b (Pou4f2) reporter (assay MGI:7444407).
Structure Cell Type Level Pattern Image Note
TS21: trigeminal ganglion trigeminal neuron Present 4C'

Specimen 4D: embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: green - Tuj1 (Tubb3); red - Brn3b (Pou4f2) reporter (assay MGI:7444407).
Structure Level Pattern Image Note
TS21: optic stalk Present 4D

Specimen 4E': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: green - Tuj1 (Tubb3); red - Brn3b (Pou4f2) reporter (assay MGI:7444407).
Structure Level Pattern Image Note
TS21: optic stalk Present 4E'

Specimen 4F': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: green - Tuj1 (Tubb3); red - Brn3b (Pou4f2) reporter (assay MGI:7444407).
Structure Level Pattern Image Note
TS21: optic nerve Present 4F'


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory