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Gene Expression Data
Assay Details
Assay
Reference: J:278612 Miltner AM, et al., A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci. 2019 Jun 14;20(12):2903
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7444407
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Reporter: mCherry
Antibody: anti-mCherry (NBP2-25157)
Assay notes: Two mCherry antibodies were described, but the one used in this assay was not detailed. Anti-mCherry (AB0040-200) MGI:6724029 may have been used in this assay.
Results
Specimen 2A: postnatal adult; Pou4f2em1Altrr/Pou4f2+ (more )
Structure Level Pattern Image Note
TS28: retina Present 2A
Specimen 2B/B': postnatal adult; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 2B' , 2B Co-localization with pan-Brn3 antibody staining. No expression in small subset of Brn3+ cells.
Specimen 2C/C': postnatal adult; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - Rbpms (assay MGI:7444410).
Structure Cell Type Level Pattern Image Note
TS28: retina retinal ganglion cell Present Regionally restricted 2C , 2C' Co-localization with Rbpms expression in retinal ganglion cells. 100% of expressing cells are Rbpms+. No expression in Rbpms+ cell bodies.
Specimen 2D/D': postnatal adult; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - Tuj1 (Tubb3 assay MGI:7444411).
Structure Cell Type Level Pattern Image Note
TS28: retina retinal ganglion cell Present Regionally restricted 2D , 2D' Co-localization with Tuj1 (Tubb3) expression in retinal ganglion cell axonal bundles.
Specimen 2E/E'/E''/E''': postnatal adult; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Triple labeled: red - Brn3b (Pou4f2) reporter; green - Pax6 (assay MGI:7444416); gray - Rpms (assay MGI:7444410).
Structure Cell Type Level Pattern Image Note
TS28: retina ganglion cell layer retinal ganglion cell Present Regionally restricted 2E , 2E'' , 2E' , 2E''' Specific expression in ganglion cell layer. Co-localization with Rbpms expression in retinal ganglion cells.
TS28: retina ganglion cell layer amacrine cell Absent 2E''' , 2E' , 2E'' , 2E No expression in Pax6+ Rbpms- amacrine cells.
Specimen 3A/A'': embryonic day 11.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Structure Level Pattern Image Note
TS19: retina Present Regionally restricted 3A , 3A'' Expression in center of retina.
Specimen 3B/B'': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Structure Cell Type Level Pattern Image Note
TS21: retina retinal ganglion cell Present Regionally restricted 3B'' , 3B Expression in retinal ganglion cell axonal fibers.
TS21: optic nerve Present 3B , 3B''
Specimen 3C/C'': postnatal day 0; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Structure Cell Type Level Pattern Image Note
TS27: retina retinal ganglion cell Present Regionally restricted 3C , 3C'' Expression in retinal ganglion cell axonal fibers.
Specimen 3D/D': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Pcna (assay MGI:7444420).
Structure Cell Type Level Pattern Image Note
TS21: retina retinal ganglion cell Present Regionally restricted 3D , 3D' Co-localization with Pcna expression in small fraction of retinal ganglion cells.
Specimen 3E/E': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Atoh7 (assay MGI:7444423).
Structure Level Pattern Image Note
TS21: retina Present Regionally restricted 3E' , 3E Co-localization with Atoh7 in subset of cells.
Specimen 3F/F': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Sncg (assay MGI:7444424).
Structure Cell Type Level Pattern Image Note
TS21: retina retinal ganglion cell Present Regionally restricted 3F , 3F' Co-localization with Sncg expression in all Sncg+ retinal ganglion cells.
Specimen 4A: postnatal day 0; Pou4f2em1Altrr/Pou4f2+ (more )
Structure Level Pattern Image Note
TS27: optic nerve Present 4A
TS27: optic tract Present 4A
Specimen 4B/B': postnatal day 0; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Structure Level Pattern Image Note
TS27: superior colliculus Present Regionally restricted 4B , 4B' Co-localization with pan-Brn3 antibody staining in superior colluclus.
Specimen 4C/C': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Structure Cell Type Level Pattern Image Note
TS21: trigeminal ganglion trigeminal neuron Present Regionally restricted 4C , 4C' Co-localization with Tuj1 expression in trigeminal ganglion. Salt-and-pepper expression in neurons.
Specimen 4D: embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Structure Level Pattern Image Note
TS21: optic stalk Present 4D
TS21: 3rd ventricle Present Regionally restricted 4D Expression in Tuj1- neuroepithelial cells in optic recess.
Specimen 4E/E': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Structure Level Pattern Image Note
TS21: optic nerve Present 4E , 4E'
TS21: optic stalk Present Regionally restricted 4E' , 4E Expression in Tuj1- cells in optic stalk
Specimen 4F/F': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Structure Level Pattern Image Note
TS21: optic nerve Present 4F' , 4F
TS21: 3rd ventricle Present Regionally restricted 4F , 4F' Expression in Tuj1- neuroepithelial cells in optic recess.
Specimen S2A/A''': embryonic day 13.5; Pou4f2em1Altrr/Pou4f2+ (more )
Note: Triple labeled: red - Brn3b (Pou4f2) reporter; green - Pax2 (assay MGI:7444425); gray - Pcna (assay MGI:7444420).
Structure Level Pattern Image Note
TS21: optic stalk Present Regionally restricted S2A''' , S2A Expression in Pax2+ Pcna+ cells in optic stalk.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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07/29/2025
MGI 6.24 		The Jackson Laboratory