Gene Expression Data
Assay Details
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Assay
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Reference:
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J:278612
Miltner AM, et al., A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci. 2019 Jun 14;20(12):2903
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7444407
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Gene symbol:
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Pou4f2
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Gene name:
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POU domain, class 4, transcription factor 2
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Reporter:
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mCherry
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Antibody:
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anti-mCherry (NBP2-25157)
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Assay notes:
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Two mCherry antibodies were described, but the one used in this assay was not detailed. Anti-mCherry (AB0040-200) MGI:6724029 may have been used in this assay.
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B/B':
postnatal adult; Pou4f2em1Altrr/Pou4f2 +
(more  )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Specimen
2B/B':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina |
Present |
Regionally restricted |
2B'
,
2B
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Co-localization with pan-Brn3 antibody staining. No expression in small subset of Brn3+ cells.
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Specimen
2C/C':
postnatal adult; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - Rbpms (assay MGI:7444410).
Specimen
2C/C':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - Rbpms (assay MGI:7444410).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2C
,
2C'
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Co-localization with Rbpms expression in retinal ganglion cells. 100% of expressing cells are Rbpms+. No expression in Rbpms+ cell bodies.
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Specimen
2D/D':
postnatal adult; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - Tuj1 (Tubb3 assay MGI:7444411).
Specimen
2D/D':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - Tuj1 (Tubb3 assay MGI:7444411).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2D
,
2D'
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Co-localization with Tuj1 (Tubb3) expression in retinal ganglion cell axonal bundles.
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Specimen
2E/E'/E''/E''':
postnatal adult; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Triple labeled: red - Brn3b (Pou4f2) reporter; green - Pax6 (assay MGI:7444416); gray - Rpms (assay MGI:7444410).
Specimen
2E/E'/E''/E''':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: red - Brn3b (Pou4f2) reporter; green - Pax6 (assay MGI:7444416); gray - Rpms (assay MGI:7444410).
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Specimen
3A/A'':
embryonic day 11.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Specimen
3A/A'':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: retina |
Present |
Regionally restricted |
3A
,
3A''
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Expression in center of retina.
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Specimen
3B/B'':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Specimen
3B/B'':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: retina |
retinal ganglion cell |
Present |
Regionally restricted |
3B''
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3B
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Expression in retinal ganglion cell axonal fibers.
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| TS21: optic nerve |
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Present |
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3B
,
3B''
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Specimen
3C/C'':
postnatal day 0; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Specimen
3C/C'':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: retina |
retinal ganglion cell |
Present |
Regionally restricted |
3C
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3C''
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Expression in retinal ganglion cell axonal fibers.
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Specimen
3D/D':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Pcna (assay MGI:7444420).
Specimen
3D/D':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Pcna (assay MGI:7444420).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: retina |
retinal ganglion cell |
Present |
Regionally restricted |
3D
,
3D'
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Co-localization with Pcna expression in small fraction of retinal ganglion cells.
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Specimen
3E/E':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Atoh7 (assay MGI:7444423).
Specimen
3E/E':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Atoh7 (assay MGI:7444423).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
3E'
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3E
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Co-localization with Atoh7 in subset of cells.
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Specimen
3F/F':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Sncg (assay MGI:7444424).
Specimen
3F/F':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Sncg (assay MGI:7444424).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: retina |
retinal ganglion cell |
Present |
Regionally restricted |
3F
,
3F'
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Co-localization with Sncg expression in all Sncg+ retinal ganglion cells.
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Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
4B/B':
postnatal day 0; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
Specimen
4B/B':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; teal - pan-Brn3 immunohistochemistry.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: superior colliculus |
Present |
Regionally restricted |
4B
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4B'
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Co-localization with pan-Brn3 antibody staining in superior colluclus.
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Specimen
4C/C':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Specimen
4C/C':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: trigeminal ganglion |
trigeminal neuron |
Present |
Regionally restricted |
4C
,
4C'
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Co-localization with Tuj1 expression in trigeminal ganglion. Salt-and-pepper expression in neurons.
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Specimen
4D:
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Specimen
4D:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
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Specimen
4E/E':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Specimen
4E/E':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
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Specimen
4F/F':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
Specimen
4F/F':
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Brn3b (Pou4f2) reporter; green - Tuj1 (Tubb3 assay MGI:7444411).
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Specimen
S2A/A''':
embryonic day 13.5; Pou4f2em1Altrr/Pou4f2 +
(more )
Note: Triple labeled: red - Brn3b (Pou4f2) reporter; green - Pax2 (assay MGI:7444425); gray - Pcna (assay MGI:7444420).
Specimen
S2A/A''':
(close )
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Genetic Background:
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involves: C57BL/6N
|
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Mutant Allele(s):
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Pou4f2em1Altrr/Pou4f2+
|
|
Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: red - Brn3b (Pou4f2) reporter; green - Pax2 (assay MGI:7444425); gray - Pcna (assay MGI:7444420).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: optic stalk |
Present |
Regionally restricted |
S2A'''
,
S2A
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Expression in Pax2+ Pcna+ cells in optic stalk.
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