Probe:	ob-r-all cDNA
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: notochord	Present		1A/B
TS16: optic cup	Weak		1A/B
TS16: central nervous system	Weak	Regionally restricted	1A/B	Expression is in the presumptive leptomeninges.
TS16: embryo	Present	Regionally restricted	1A/B	Expression is in the notochord, optic cup, and in the presumptive leptomeninges and is absent everywhere else.

Structure	Level	Pattern	Image	Note
TS20: notochord	Strong		1C/D
TS20: trunk mesenchyme	Present	Regionally restricted	1C/D	Expression is in the condensing mesenchyme surrounding the spinal cord.
TS20: skeleton	Present	Regionally restricted	1C/D	Expression is in the condensing mesenchyme of the long bones.
TS20: face mesenchyme	Present		1C/D

Structure	Level	Pattern	Image	Note
TS20: liver	Moderate		1E/F

Structure	Level	Pattern	Image	Note
TS22: face mesenchyme	Present		2A/B
TS22: leptomeninges	Present		2A/B
TS22: tooth mesenchyme	Present	Regionally restricted	2A/B	Expression is in the condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: radius	Present		2D/E
TS24: ulna	Present		2D/E
TS24: forearm mesenchyme	Present	Regionally restricted	2D/E	Expression is in the connective tissue surrounding the bones.

Structure	Level	Pattern	Image	Note
TS24: alveolus epithelium	Weak		2G/H
TS24: bronchus epithelium	Weak		2G/H

Structure	Level	Pattern	Image	Note
TS24: choroid plexus	Strong		2J/K
TS24: leptomeninges	Strong		2J/K

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lung mesenchyme		Present	Regionally restricted		Expression is in the mesenchyme surrounding the major bronchi.
TS22: central nervous system		Present	Regionally restricted		Expression is in the leptomeninges and the epithelial cells that make up the choroid plexus and is absent in neurons and glia.
TS22: choroid plexus		Strong
TS22: leptomeninges		Strong
TS22: liver		Absent
TS22: central nervous system	glial cell	Absent
TS22: central nervous system	neuron	Absent

Structure	Level	Pattern	Image	Note
TS24: embryo mesenchyme	Strong
TS24: face mesenchyme	Strong
TS24: periosteum	Strong
TS24: hair follicle	Present
TS24: intestine	Weak
TS24: renal interstitium	Weak
TS24: spleen	Weak
TS24: cortical early distal tubule	Absent
TS24: cortical early proximal tubule	Absent
TS24: maturing glomerular tuft	Absent

Structure	Level	Pattern	Image	Note
TS26: choroid plexus	Strong
TS26: leptomeninges	Strong
TS26: tooth mesenchyme	Strong
TS26: bone	Present
TS26: embryo mesenchyme	Present
TS26: hair follicle	Present