Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:333149
Zhao T, et al., Sertoli cell PUMILIO proteins modulate mouse testis size through translational control of cell cycle regulators. Biol Reprod. 2022 Jul 25;107(1):135-147
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7437576
|
|
Gene symbol:
|
Cdkn1b
|
|
Gene name:
|
cyclin dependent kinase inhibitor 1B
|
|
Antibody:
|
anti-p27 KIP1 [EPFHCR16] (ab92741)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
Fixation was with Hartman's fixative.
|
|
|
Results
|
Specimen
S2 CDKN1B P14 Control:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Pum1tm1.1Eyx/Pum1tm1.1Eyx
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
|
S2_CDKN1B P14 Control
|
|
Specimen
S2 CDKN1B P14 AcP1KO:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Conditional mutant.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Strong |
|
S2_CDKN1B P14 AcP1KO
|
Increase in Sertoli cell expression compared to control.
|
Specimen
S2 CDKN1B Adult Control:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Pum1tm1.1Eyx/Pum1tm1.1Eyx
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
|
S2_CDKN1B Adult Control
|
|
Specimen
S2 CDKN1B Adult AcP1KO:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Conditional mutant.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Strong |
|
S2_CDKN1B Adult AcP1KO
|
Increase in Sertoli cell expression compared to control.
|
Specimen
5E CDKN1B Control:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Pum1tm1.1Eyx/Pum1tm1.1Eyx
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
|
5E CDKN1B Control
|
|
Specimen
5E CDKN1B AcP1KO:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6 * SJL
|
|
Mutant Allele(s):
|
Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Conditional mutant.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Strong |
|
5E CDKN1B AcP1KO
|
Increase in Sertoli cell expression compared to control.
|
Specimen
5E CDKN1B AcP1KO; Cdkn1b+/-:
(close )
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Weak |
|
5E CDKN1B AcP1KO; Cdkn1b+/-
|
Expression in Sertoli cells reduced compared to control.
|
|
)
Analysis Tools