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Gene Expression Data
Assay Details
Assay
Reference: J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7434576
Gene symbol: Cdh2
Gene name: cadherin 2
Antibody: anti-N-cadherin (clone 32; 610920/610921)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 8A: embryonic day 13.5 (more )
Note: Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS21: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8A Expressed in the apical junctional complex of neuroepithelium at the ventricular surface continuously.

Specimen 8B: embryonic day 13.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS21: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8B Partially lost at the ventricular surface.

Specimen 8E: embryonic day 16.5 (more )
Note: Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS24: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8E Expressed at the ventricular surface continuously.

Specimen 8F: embryonic day 16.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS24: cerebellum ventricular layer neuroepithelial stem cell Absent 8F No expression at the ventricular surface.

Specimen 8M: embryonic day 16.5 (more )
Note: Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS24: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8M Expressed at the ventricular surface continuously.

Specimen 8N: embryonic day 16.5; Tg(GFAP-cre)25Mes/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS24: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8N Loss of apical localization of Cdh2 correlated with the Yap1-reduced area.

Specimen S8A: postnatal day 7 (more )
Note: Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum external granule cell layer cerebellar granule cell Present S8A left , S8A right , S8A merge Localized at the cell junction.
TS28: cerebellum external granule cell layer cerebellar granule cell precursor Present S8A left , S8A right , S8A merge Localized at the cell junction.

Specimen S8B: postnatal day 7; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: green - Cdh2; red - Pals1 (assay MGI:7434577).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum external granule cell layer cerebellar granule cell Present S8B merge , S8B right , S8B left Localized at the cell junction, similar as in the wild-type.
TS28: cerebellum external granule cell layer cerebellar granule cell precursor Present S8B merge , S8B right , S8B left Localized at the cell junction, similar as in the wild-type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory