Probe:	pBRAH16
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Graded	4C	Expressed in a decreasing caudal-to-rostral intensity in the ventral region.
TS17: diencephalon	Present	Regionally restricted	4C	Expressed at the prosomere 2/3 boundary separating the telencephalon from the diencephalon.
TS17: eye	Present		4C
TS17: olfactory placode	Present		4C
TS17: otocyst	Present		4C
TS17: rhombomere	Present	Regionally restricted	4C	Rostral boundary approximately at the second rhombomere.
TS17: dorsal root ganglion	Absent		4C

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4D	Expressed in the ventricular zone near the ventral midline.
TS17: dorsal root ganglion	Absent		4D

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	4G	Expression was largely confined to the ventral ventricular region near the ventral midline.
TS20: dorsal root ganglion	Absent		4G

Structure	Level	Pattern	Image	Note
TS22: spinal cord mantle layer	Present	Scattered	4K	Expressed in cells migrated from ventral to dorsal regions.
TS22: spinal cord ventricular layer	Present	Regionally restricted	4K	Expressed near the ventral midline.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Graded	9D	Expressed in a decreasing caudal-to-rostral pattern.
TS17: rhombomere	Present	Regionally restricted	9D	Expression terminates rostrally approximately at the second rhombomere.
TS17: infundibular recess of 3rd ventricle	Present		9D
TS17: olfactory placode	Present		9D
TS17: diencephalon	Present	Regionally restricted	9D	Expressed at the prosomere 2/3 boundary separating the telencephalon from the diencephalon.
TS17: telencephalon	Present	Regionally restricted	9D	Expressed in the ventral region.

Structure	Level	Pattern	Image	Note
TS17: brain	Absent		9E
TS17: neural tube	Absent		9E
TS17: olfactory placode	Present		9E