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Gene Expression Data
Assay Details
Assay
Reference: J:231673 Gustin SE, et al., WNT/beta-catenin and p27/FOXL2 differentially regulate supporting cell proliferation in the developing ovary. Dev Biol. 2016 Apr 15;412(2):250-60
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7432735
Gene symbol: Lgr5
Gene name: leucine rich repeat containing G protein coupled receptor 5
Reporter: GFP
Antibody: anti-EGFP/EYFP
Results
Specimen 4A: embryonic day 13.5; Lgr5tm1(cre/ERT2)Cle/Lgr5+ (more )
Note: Triple labeled: green - Lgr5 reporter; red - Cdkn1b (assay MGI:7432672); white - Gata4 (assay MGI:7432676).
Structure Level Pattern Image Note
TS21: ovary Present Regionally restricted 4A iii , 4A i , 4A ii , 4A left (i) GATA4-positive/LGR5GFP-positive/Cdkn1b-negative cells (green cells marked by >), (ii) GATA4-positive/LGR5GFP-positive/Cdkn1b-positive cells (yellow cells marked by arrows) and (iii) GATA4-positive/LRG5GFP-negative/Cdkn1b-positive cells (red cells). Triple positive cells appeared to be populating the medulla of the ovary.
Specimen 4B: embryonic day 16.5; Lgr5tm1(cre/ERT2)Cle/Lgr5+ (more )
Note: Triple labeled: green - Lgr5 reporter; red - Cdkn1b (assay MGI:7432672); white - Gata4 (assay MGI:7432676).
Structure Level Pattern Image Note
TS24: ovary Present Regionally restricted 4B iii , 4B i , 4B left , 4B ii Although some GATA4-positive/p27-positive/Lgr5-GFP-positive cells were also detected in E16.5 ovaries, there was a distinct demarcation between GATA4-positive/p27-negative/Lgr5-GFP-positive cells located in the cortex and the GATA4-positive/p27-positive/Lgr5-GFP-negative cells located in the medulla.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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