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Gene Expression Data
Assay Details
Assay
Reference: J:302455 Stupka N, et al., Versican processing by a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats proteinases-5 and -15 facilitates myoblast fusion. J Biol Chem. 2013 Jan 18;288(3):1907-17
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7432550
Gene symbol: Vcan
Gene name: versican
Antibody: anti-DPEAAE
Detection system: Secondary antibody coupled to fluorescein
Assay notes: The antibody specifically detects the ADAMTS cleavage site in the V0 and V1 splice variants of Versican.
Results
Specimen 1A wildtype E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hindlimb muscle Present 1A top left
Specimen 1A Adamts5-/- E13.5: embryonic day 13.5; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Structure Level Pattern Image Note
TS21: hindlimb muscle Present 1A top right
Specimen 1A wildtype postnatal: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: skeletal muscle Present 1A lower left
Specimen 1A Adamts5-/- postnatal: postnatal week 3; Adamts5tm1Dgen/Adamts5tm1Dgen (more )
Structure Level Pattern Image Note
TS28: skeletal muscle Absent 1A lower right
Specimen 1E top: postnatal adult (more )
Note: Double labeled: green - Vcan; red - Des (assay MGI:7432566).
Structure Cell Type Level Pattern Image Note
TS28: skeletal muscle Present Regionally restricted 1E Merge top , 1E DPEAAE top Versican cleavage co-localized with Desmin in skeletal muscle fibers.
TS28: skeletal muscle skeletal muscle satellite cell Present 1E Merge top , 1E DPEAAE top Versican cleavage co-localized with Desmin in skeletal muscle satellite cells.
Specimen 1E lower: postnatal adult (more )
Note: Double labeled: green - Vcan; red - Des (assay MGI:7432566).
Structure Cell Type Level Pattern Image Note
TS28: skeletal muscle Present Regionally restricted 1E DPEAAE lower , 1E Merge lower Versican cleavage co-localized with Desmin in skeletal muscle fibers.
TS28: skeletal muscle skeletal muscle satellite cell Present 1E Merge lower , 1E DPEAAE lower Versican cleavage co-localized with Desmin in skeletal muscle satellite cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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