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Gene Expression Data
Assay Details
Assay
Reference: J:161290 Chizhikov VV, et al., Lmx1a regulates fates and location of cells originating from the cerebellar rhombic lip and telencephalic cortical hem. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10725-30
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7431459
Gene symbol: Atoh1
Gene name: atonal bHLH transcription factor 1
Antibody: Math1 antibody
Assay notes: Two antibodies were described; either MGI:3056756 or MGI:7431213 were used in this assay.
Results
Specimen 1D: embryonic day 12.5 (more )
Note: Double labeled: green - Atoh1; red - Lmx1a (assay MGI:7431456).
Structure Level Pattern Image Note
TS20: rhombic lip Present Regionally restricted 1e''' , 1e'' , 1D , 1E Many Lmx1a+/Atoh1- cells were noted. Rare Lmx1a/Atoh1 double positive cells were seen.
Specimen 1G: embryonic day 16.5 (more )
Note: Double labeled: green - Atoh1; red - Lmx1a (assay MGI:7431456).
Structure Level Pattern Image Note
TS24: rhombic lip Present Regionally restricted 1g''' , 1G , 1g'' Many Lmx1a+/Atoh1- cells were noted. Rare Lmx1a/Atoh1 double positive cells were seen.
Specimen 4B: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Lmx1a-EGFP/cre)3Kjmi/0 (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS21: cerebellum cerebellar granule cell precursor Present 4B Expression was detected in granule cell precursors. Beta-gal labeled cells co-expressed Atoh1.
Specimen 4D: embryonic day 13.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Lmx1adr/Lmx1adr, Tg(Lmx1a-EGFP/cre)3Kjmi/0 (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS21: cerebellum cerebellar granule cell precursor Present Regionally restricted 4D Expression was detected in granule cell precursors in the mutant. Beta-gal labeled cells co-expressed Atoh1.
Specimen 4F: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Lmx1a-EGFP/cre)3Kjmi/0 (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS26: cerebellum external granule cell layer cerebellar granule cell precursor Present 4F Beta-gal labeled cells co-expressed Atoh1.
Specimen 4H: embryonic day 18.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Lmx1adr/Lmx1adr, Tg(Lmx1a-EGFP/cre)3Kjmi/0 (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS26: cerebellum external granule cell layer cerebellar granule cell precursor Present 4H Beta-gal labeled cells co-expressed Atoh1. The double labeled cells extend much farther into the anterior cerebellum of the mutant compared to wild type.
Specimen 4i': postnatal day 3; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Tg(Lmx1a-EGFP/cre)3Kjmi/0 (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS27: cerebellum external granule cell layer cerebellar granule cell Present 4i' Beta-gal labeled cells co-expressed Atoh1.
Specimen 4j': postnatal day 3; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Lmx1adr/Lmx1adr, Tg(Lmx1a-EGFP/cre)3Kjmi/0 (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS27: cerebellum external granule cell layer cerebellar granule cell Present 4j' , 4j'' Beta-gal labeled cells co-expressed Atoh1. The double labeled cells extend much farther into the anterior cerebellum of the mutant compared to wild type.
Specimen S4B: postnatal day 3; Gdf7tm3(cre)Tmj/Gdf7+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS27: cerebellum external granule cell layer cerebellar granule cell Present S4B No co-expression with beta-galactosidase was detected in the wild type.
Specimen S4C: postnatal day 3; Gdf7tm3(cre)Tmj/Gdf7+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Lmx1adr/Lmx1adr (more )
Note: Conditional mutant. Double labeled: red - Atoh1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS27: cerebellum external granule cell layer cerebellar granule cell Present S4C In dreher mice, some beta-gal+ cells were detected co-expressing with Atoh1 in granule cells.
Specimen S5B: embryonic day 13.75 (more )
Note: Double labeled: green - Atoh1; red - Lmx1a (assay MGI:7431456).
Structure Level Pattern Image Note
TS21: rhombic lip Present S5C , S5B Some Atoh1+/Lmx1a+ cells were noted.
Specimen S5E: embryonic day 13.75; Lmx1adr/Lmx1adr (more )
Note: Double labeled: green - Atoh1; red - Lmx1a (assay MGI:7431456).
Structure Level Pattern Image Note
TS21: rhombic lip Present S5F , S5E Many more Lmx1a+ cells coexpress Atoh1 in dreher rhombic lip than in wild type.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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