Antibody:	Anti-TFF3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS24: oral epithelium	stratified squamous epithelial cell	Moderate		1A	mild to moderate

Structure	Cell Type	Level	Pattern	Image	Note
TS26: enamel epithelium		Present		1B
TS26: stellate reticulum		Present		1B
TS26: dental papilla	odontoblast	Present	Regionally restricted	1B	Expressed in odontoblasts.

Structure	Level	Pattern	Image	Note
TS23: stomach epithelium	Weak		1C	Discrete expression in the undifferentiated endodermal epithelium.

Structure	Level	Pattern	Image	Note
TS26: stomach mucosa	Present		1D	Expressed in all sections, especially in the differentiated nonglandular stomach.

Structure	Level	Pattern	Image	Note
TS23: small intestine epithelium	Moderate	Scattered	1E	Mild expression in the endodermal epithelium undergoing differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: small intestine epithelium	small intestine goblet cell	Present		1F

Structure	Cell Type	Level	Pattern	Image	Note
TS25: colon epithelium	colon goblet cell	Strong		1G

Structure	Cell Type	Level	Pattern	Image	Note
TS24: nasal cavity respiratory epithelium	ciliated epithelial cell	Present		2A
TS24: nasal cavity respiratory epithelium	nasal mucosa goblet cell	Present		2A

Structure	Level	Pattern	Image	Note
TS22: bronchiole epithelium	Weak	Regionally restricted	2B	Expressed only in several sections.
TS22: lobar bronchus epithelium	Weak	Regionally restricted	2B	Expressed only in several sections.

Structure	Level	Pattern	Image	Note
TS25: lobar bronchus epithelium	Present		2C
TS25: bronchiole epithelium	Present	Regionally restricted	2C	Expression was not observed in small bronchiolar branches.

Structure	Level	Pattern	Image	Note
TS25: renal convoluted tubule	Present		2D	Moderate to strong.
TS25: collecting duct	Present		2D
TS25: loop of Henle	Present	Regionally restricted	2D	Expressed in the thick limb.
TS25: maturing glomerular tuft	Absent		2D
TS25: nephrogenic zone	Absent		2D

Structure	Level	Pattern	Image	Note
TS23: epidermis	Moderate	Diffuse	2E	Mild to moderate.

Structure	Level	Pattern	Image	Note
TS26: epidermis granular layer	Present		2F
TS26: epidermis spinous layer	Present		2F
TS26: hair follicle	Present		2F
TS26: epidermis cornified layer	Trace		2F	Only occasionally.
TS26: dermis	Absent
TS26: epidermis basal layer	Absent

Structure	Level	Pattern	Image	Note
TS23: conjunctiva epithelium	Moderate		2G
TS23: cornea epithelium	Moderate		2G
TS23: lens	Moderate		2G

Structure	Level	Pattern	Image	Note
TS25: cochlea epithelium	Present		2H
TS25: cochlear ganglion	Present		2H

Structure	Cell Type	Level	Pattern	Image	Note
TS28: small intestine epithelium	small intestine goblet cell	Strong		3A

Structure	Cell Type	Level	Pattern	Image	Note
TS22: oral epithelium	stratified squamous epithelial cell	Weak			Although still visible, expression was the weakest.
TS22: gut mucosa		Weak			Weak or no immunostaining was detected.
TS22: nasal cavity respiratory epithelium		Moderate	Scattered		Sporadically detected.
TS22: renal tubule		Trace
TS22: bladder		Absent
TS22: dermis		Absent
TS22: maturing glomerular tuft		Absent
TS22: nephrogenic zone		Absent
TS22: epidermis		Moderate	Diffuse		Mild to moderate.
TS22: conjunctiva epithelium		Moderate
TS22: cornea epithelium		Moderate
TS22: lens		Moderate
TS22: optic nerve		Moderate
TS22: retina		Moderate

Structure	Cell Type	Level	Pattern	Image	Note
TS23: oral epithelium	stratified squamous epithelial cell	Moderate			Mild to moderate.
TS23: nasal cavity respiratory epithelium		Moderate			Sporadically detected.
TS23: bronchiole epithelium		Weak	Regionally restricted		Expressed only in several sections.
TS23: lobar bronchus epithelium		Weak	Regionally restricted		Expressed only in several sections.
TS23: collecting duct		Weak	Regionally restricted
TS23: cortical renal tubule		Weak	Regionally restricted
TS23: medullary renal tubule		Weak	Regionally restricted
TS23: bladder		Absent
TS23: dermis		Absent
TS23: maturing glomerular tuft		Absent
TS23: nephrogenic zone		Absent
TS23: epidermis		Moderate	Diffuse		Mild to moderate.
TS23: optic nerve		Moderate
TS23: retina		Moderate

Structure	Cell Type	Level	Pattern	Image	Note
TS24: stomach mucosa		Present
TS24: small intestine epithelium	small intestine goblet cell	Strong
TS24: colon epithelium	colon goblet cell	Present			The goblet cells were not completely differentiated, thus the signal was not consistent in all sections.
TS24: bronchiole epithelium		Weak	Regionally restricted		Expressed only in several sections.
TS24: lobar bronchus epithelium		Weak	Regionally restricted		Expressed only in several sections.
TS24: cortical renal tubule		Present
TS24: medullary renal tubule		Present
TS24: bladder		Absent
TS24: maturing glomerular tuft		Absent
TS24: nephrogenic zone		Absent
TS24: conjunctiva epithelium		Moderate
TS24: cornea epithelium		Moderate
TS24: lens		Moderate
TS24: optic nerve		Moderate
TS24: retina		Moderate

Structure	Cell Type	Level	Pattern	Image	Note
TS25: oral epithelium	stratified squamous epithelial cell	Moderate			Mild to moderate
TS25: stomach mucosa		Present			Expressed in all sections, especially in the nonglandular stomach.
TS25: stomach glandular region mucosa		Weak			Expression was weak or missing.
TS25: rectum epithelium		Strong
TS25: nasal cavity respiratory epithelium	ciliated epithelial cell	Present
TS25: nasal cavity respiratory epithelium	nasal mucosa goblet cell	Present
TS25: bladder		Absent
TS25: conjunctiva epithelium		Moderate			Mild to moderate.
TS25: cornea epithelium		Moderate			Mild to moderate.
TS25: lens		Moderate			Mild to moderate.
TS25: optic nerve		Moderate			Mild to moderate.
TS25: retina		Moderate			Mild to moderate.
TS25: semicircular canal epithelium		Present

Structure	Cell Type	Level	Pattern	Image	Note
TS26: oral epithelium	stratified squamous epithelial cell	Moderate			Mild to moderate.
TS26: stomach glandular region mucosa		Weak			Expression was weak or missing.
TS26: small intestine epithelium	small intestine goblet cell	Strong
TS26: colon epithelium	colon goblet cell	Strong
TS26: rectum epithelium		Strong
TS26: nasal cavity respiratory epithelium	ciliated epithelial cell	Present
TS26: nasal cavity respiratory epithelium	nasal mucosa goblet cell	Present
TS26: collecting duct		Present
TS26: lobar bronchus epithelium		Present
TS26: loop of Henle		Present
TS26: renal convoluted tubule		Present
TS26: bronchiole epithelium		Present	Regionally restricted		Expression was not observed in small bronchiolar branches.
TS26: bladder		Absent
TS26: glomerular tuft		Absent
TS26: nephrogenic zone		Absent
TS26: conjunctiva epithelium		Moderate			Mild to moderate.
TS26: cornea epithelium		Moderate			Mild to moderate.
TS26: lens		Moderate			Mild to moderate.
TS26: optic nerve		Moderate			Mild to moderate.
TS26: retina		Moderate			Mild to moderate.