About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:284573 Gao XL, et al., High-mobility group nucleosomal binding domain 2 protects against microcephaly by maintaining global chromatin accessibility during corticogenesis. J Biol Chem. 2020 Jan 10;295(2):468-480
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7428971
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti PAX6 (DSHB)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Three antibodies were listed MGI:1928078, MGI:7428931, and MGI:2157328, but the antibody used in this assay was not detailed.
Results
Specimen 4G WT: embryonic day 18.5 (more )
Note: Triple labeled: red - Pax6; green - Eomes (assay MGI:7428954); gray - Bcl11b (assay MGI:7428968).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex forebrain radial glial cell Present 4G WT
TS26: cerebral cortex Present Regionally restricted 4G WT Co-expression with Eomes was noted in transitional cell type.
Specimen 4G mKO: embryonic day 18.5; Hmgn2tm1a(KOMP)Wtsi/Hmgn2tm1a(KOMP)Wtsi (more )
Note: Triple labeled: red - Pax6; green - Eomes (assay MGI:7428954); gray - Bcl11b (assay MGI:7428968).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex forebrain radial glial cell Present 4G mKO The immunopositive retinal glial pool did not change in mutant compared with wild type.
TS26: cerebral cortex Present Regionally restricted 4G mKO The transitional cell type (Eomes+Pax6+) pool expanded in the mutant compared with wild type. In addition, ectopic transitional cells were noted outside the ventricular zone-subventricular zone in mutant mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory