Antibody:	Anti-Tbr2 (AB15894)
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Three antibodies were listed MGI:6153996, MGI:5587805, and MGI:6437867, but the antibody used in this assay was not detailed.

Structure	Level	Pattern	Image	Note
TS23: lateral ganglionic eminence	Present	Regionally restricted	1E2	Expression co-localized with Hmgn2 in intermediate progenitor cells.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	4G WT	Expression was detected in intermediate progenitor cells; co-expression with Pax6 was noted in transitional cell type.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	4G mKO	Expression expanded in intermediate progenitor cells in the mutant compared with wild type; the transitional cell type (Eomes+Pax6+) pool expanded in the mutant compared with wild type. In addition, ectopic intermediate progenitor cells and transitional cells were noted outside the ventricular zone-subventricular zone in mutant mice.