About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:284573 Gao XL, et al., High-mobility group nucleosomal binding domain 2 protects against microcephaly by maintaining global chromatin accessibility during corticogenesis. J Biol Chem. 2020 Jan 10;295(2):468-480
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7428941
Gene symbol: Hmgn2
Gene name: high mobility group nucleosomal binding domain 2
Antibody: Anti-HMGN2 (D9B9) #9437
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were listed MGI:7428885 and MGI:7428888; either could have been used in this assay.
Results
Specimen 1C E11.5 Cortex: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 1C E11.5 center
TS20: cerebral cortex ventricular layer Present 1C E11.5 center
TS20: cortical plate Present 1C E11.5 center
Specimen 1C E11.5 GE: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS20: ganglionic eminence Present 1C E11.5 right
Specimen 1C E13-14 Cortex: embryonic day 13.0-14.0 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex subventricular zone Present 1C E13-14 center
TS21: cerebral cortex ventricular layer Present 1C E13-14 center
TS21: cortical plate Present 1C E13-14 center
Specimen 1C E13-14 GE: embryonic day 13.0-14.0 (more )
Structure Level Pattern Image Note
TS21: ganglionic eminence Present 1C E13-14 right
Specimen 1C E15-16 Cortex: embryonic day 15.0-16.0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 1C E15-16 center
TS23: cerebral cortex ventricular layer Present 1C E15-16 center
TS23: cortical plate Present 1C E15-16 center
Specimen 1C E15-16 GE: embryonic day 15.0-16.0 (more )
Structure Level Pattern Image Note
TS23: ganglionic eminence Present 1C E15-16 right
Specimen 1C E17-18 Cortex: embryonic day 17.0-18.0 (more )
Structure Level Pattern Image Note
TS25: cerebral cortex subventricular zone Present 1C E17-18 center
TS25: cerebral cortex ventricular layer Present 1C E17-18 center
TS25: cortical plate Present 1C E17-18 center
Specimen 1C E17-18 GE: embryonic day 17.0-18.0 (more )
Structure Level Pattern Image Note
TS25: ganglionic eminence Present 1C E17-18 right
Specimen 1E1: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Fut4 (assay MGI:7428952).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex ventricular layer forebrain radial glial cell Present 1E1 Expression co-localized with Fut4 in radial glia cells.
Specimen 1E2: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Eomes (assay MGI:7428954).
Structure Level Pattern Image Note
TS23: cerebral cortex Present Regionally restricted 1E2 Expression co-localized with Eomes in intermediate progenitor cells.
Specimen 1E3: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Dcx (assay MGI:7428953).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex neuron Present Regionally restricted 1E3 Expression co-localized with Dcx in immature neurons.
Specimen 1E4: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Fut4 (assay MGI:7428952).
Structure Cell Type Level Pattern Image Note
TS23: lateral ganglionic eminence forebrain radial glial cell Present 1E4 Expression co-localized with Fut4 in radial glia cells.
Specimen 1E5: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Ascl1 (assay MGI:7428955).
Structure Level Pattern Image Note
TS23: lateral ganglionic eminence Present Regionally restricted 1E5 Expression co-localized with Ascl1 in intermediate progenitor cells.
Specimen 1E6: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Dcx (assay MGI:7428953).
Structure Cell Type Level Pattern Image Note
TS23: lateral ganglionic eminence neuron Present Regionally restricted 1E6 Expression co-localized with Dxc in immature neurons.
Specimen 2B Het: embryonic day 10.5; Hmgn2tm1a(KOMP)Wtsi/Hmgn2+ (more )
Structure Level Pattern Image Note
TS17: telencephalon Present Regionally restricted 2B Het Expression was detected in the cerebral cortex.
Specimen 2B KO: embryonic day 10.5; Hmgn2tm1a(KOMP)Wtsi/Hmgn2tm1a(KOMP)Wtsi (more )
Structure Level Pattern Image Note
TS17: telencephalon Absent 2B KO Some autofluorescence from blood vessels and pia was noted.
Specimen S1E SVZ P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: telencephalon subventricular zone Present S1E P1 SVZ
Specimen S1E SVZ P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E P7 SVZ
Specimen S1E SVZ P10: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E P10 SVZ
Specimen S1E SVZ P14: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E P14 SVZ
Specimen S1E SVZ Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E Adult SVZ
Specimen S1E DG P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: dentate gyrus subgranular zone Present S1E P1 DG
Specimen S1E DG P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E P7 DG
Specimen S1E DG P10: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E P10 DG
Specimen S1E DG P14: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E P14 DG
Specimen S1E DG Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E Adult DG
Specimen S1F SVZ: postnatal adult (more )
Note: Triple labeled: green - Hmgn2; red - Gfap (assay MGI:7429204); magenta - Ncam1 (assay MGI:7429203).
Structure Cell Type Level Pattern Image Note
TS28: telencephalon subventricular zone neuron Present Regionally restricted S1F SVZ Expression co-localized with Ncam1 in immature neurons.
TS28: telencephalon subventricular zone glial cell Present Regionally restricted S1F SVZ Expression co-localized with Gfap in glial cells.
Specimen S1F SGZ: postnatal adult (more )
Note: Triple labeled: green - Hmgn2; red - Gfap (assay MGI:7429204); magenta - Ncam1 (assay MGI:7429203).
Structure Cell Type Level Pattern Image Note
TS28: dentate gyrus subgranular zone neuron Present Regionally restricted S1F SGZ Expression co-localized with Ncam1 in immature neurons.
TS28: dentate gyrus subgranular zone glial cell Present Regionally restricted S1F SGZ Expression co-localized with Gfap in glial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory