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Gene Expression Data
Assay Details
Assay
Reference: J:284573 Gao XL, et al., High-mobility group nucleosomal binding domain 2 protects against microcephaly by maintaining global chromatin accessibility during corticogenesis. J Biol Chem. 2020 Jan 10;295(2):468-480
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7428941
Gene symbol: Hmgn2
Gene name: high mobility group nucleosomal binding domain 2
Antibody: Anti-HMGN2 (D9B9) #9437
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were listed MGI:7428885 and MGI:7428888; either could have been used in this assay.
Results
Specimen 1C E11.5 Cortex: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex subventricular zone Present 1C E11.5 center
TS20: cerebral cortex ventricular layer Present 1C E11.5 center
TS20: cortical plate Present 1C E11.5 center
Specimen 1C E11.5 GE: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS20: ganglionic eminence Present 1C E11.5 right
Specimen 1C E13-14 Cortex: embryonic day 13.0-14.0 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex subventricular zone Present 1C E13-14 center
TS21: cerebral cortex ventricular layer Present 1C E13-14 center
TS21: cortical plate Present 1C E13-14 center
Specimen 1C E13-14 GE: embryonic day 13.0-14.0 (more )
Structure Level Pattern Image Note
TS21: ganglionic eminence Present 1C E13-14 right
Specimen 1C E15-16 Cortex: embryonic day 15.0-16.0 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 1C E15-16 center
TS23: cerebral cortex ventricular layer Present 1C E15-16 center
TS23: cortical plate Present 1C E15-16 center
Specimen 1C E15-16 GE: embryonic day 15.0-16.0 (more )
Structure Level Pattern Image Note
TS23: ganglionic eminence Present 1C E15-16 right
Specimen 1C E17-18 Cortex: embryonic day 17.0-18.0 (more )
Structure Level Pattern Image Note
TS25: cerebral cortex subventricular zone Present 1C E17-18 center
TS25: cerebral cortex ventricular layer Present 1C E17-18 center
TS25: cortical plate Present 1C E17-18 center
Specimen 1C E17-18 GE: embryonic day 17.0-18.0 (more )
Structure Level Pattern Image Note
TS25: ganglionic eminence Present 1C E17-18 right
Specimen 1E1: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Fut4 (assay MGI:7428952).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex ventricular layer forebrain radial glial cell Present 1E1 Expression co-localized with Fut4 in radial glia cells.
Specimen 1E2: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Eomes (assay MGI:7428954).
Structure Level Pattern Image Note
TS23: cerebral cortex Present Regionally restricted 1E2 Expression co-localized with Eomes in intermediate progenitor cells.
Specimen 1E3: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Dcx (assay MGI:7428953).
Structure Cell Type Level Pattern Image Note
TS23: cerebral cortex neuron Present Regionally restricted 1E3 Expression co-localized with Dcx in immature neurons.
Specimen 1E4: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Fut4 (assay MGI:7428952).
Structure Cell Type Level Pattern Image Note
TS23: lateral ganglionic eminence forebrain radial glial cell Present 1E4 Expression co-localized with Fut4 in radial glia cells.
Specimen 1E5: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Ascl1 (assay MGI:7428955).
Structure Level Pattern Image Note
TS23: lateral ganglionic eminence Present Regionally restricted 1E5 Expression co-localized with Ascl1 in intermediate progenitor cells.
Specimen 1E6: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Hmgn2; red - Dcx (assay MGI:7428953).
Structure Cell Type Level Pattern Image Note
TS23: lateral ganglionic eminence neuron Present Regionally restricted 1E6 Expression co-localized with Dxc in immature neurons.
Specimen 2B Het: embryonic day 10.5; Hmgn2tm1a(KOMP)Wtsi/Hmgn2+ (more )
Structure Level Pattern Image Note
TS17: telencephalon Present Regionally restricted 2B Het Expression was detected in the cerebral cortex.
Specimen 2B KO: embryonic day 10.5; Hmgn2tm1a(KOMP)Wtsi/Hmgn2tm1a(KOMP)Wtsi (more )
Structure Level Pattern Image Note
TS17: telencephalon Absent 2B KO Some autofluorescence from blood vessels and pia was noted.
Specimen S1E SVZ P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: telencephalon subventricular zone Present S1E P1 SVZ
Specimen S1E SVZ P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E P7 SVZ
Specimen S1E SVZ P10: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E P10 SVZ
Specimen S1E SVZ P14: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E P14 SVZ
Specimen S1E SVZ Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present S1E Adult SVZ
Specimen S1E DG P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: dentate gyrus subgranular zone Present S1E P1 DG
Specimen S1E DG P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E P7 DG
Specimen S1E DG P10: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E P10 DG
Specimen S1E DG P14: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E P14 DG
Specimen S1E DG Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: dentate gyrus subgranular zone Present S1E Adult DG
Specimen S1F SVZ: postnatal adult (more )
Note: Triple labeled: green - Hmgn2; red - Gfap (assay MGI:7429204); magenta - Ncam1 (assay MGI:7429203).
Structure Cell Type Level Pattern Image Note
TS28: telencephalon subventricular zone neuron Present Regionally restricted S1F SVZ Expression co-localized with Ncam1 in immature neurons.
TS28: telencephalon subventricular zone glial cell Present Regionally restricted S1F SVZ Expression co-localized with Gfap in glial cells.
Specimen S1F SGZ: postnatal adult (more )
Note: Triple labeled: green - Hmgn2; red - Gfap (assay MGI:7429204); magenta - Ncam1 (assay MGI:7429203).
Structure Cell Type Level Pattern Image Note
TS28: dentate gyrus subgranular zone neuron Present Regionally restricted S1F SGZ Expression co-localized with Ncam1 in immature neurons.
TS28: dentate gyrus subgranular zone glial cell Present Regionally restricted S1F SGZ Expression co-localized with Gfap in glial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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