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Gene Expression Data
Assay Details
Assay
Reference: J:199435 Barraud P, et al., Olfactory ensheathing glia are required for embryonic olfactory axon targeting and the migration of gonadotropin-releasing hormone neurons. Biol Open. 2013 Jul 15;2(7):750-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427814
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The authors used two Tubb3 antibodies; it is possible that another antibody (MGI:7427730) was used to generate some of these data.
Results
Specimen 1A: embryonic day 10.5 (more )
Note: Parasagittal section. Double labeled: green - Tubb3; purple - Sox10 (assay MGI:7427749).
Structure Cell Type Level Pattern Image Note
TS18: olfactory epithelium neuron Present 1B , 1B2
TS18: olfactory migratory mass neuron Present 1B , 1B2
Specimen 1C: embryonic day 11.5 (more )
Note: Parasagittal section. Double labeled: green - Tubb3; purple - Sox10 (assay MGI:7427749).
Structure Cell Type Level Pattern Image Note
TS20: olfactory nerve Present 1E1 , 1D1 , 1D2
TS20: olfactory epithelium neuron Present 1D1 , 1D2
TS20: vomeronasal organ epithelium neuron Present 1D1 , 1D2
Specimen 1G: embryonic day 12.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; purple - Sox10 (assay MGI:7427749).
Structure Cell Type Level Pattern Image Note
TS21: olfactory nerve Present 1H , 1I
TS21: vomeronasal branch of olfactory I nerve Present 1J , 1H
TS21: olfactory epithelium neuron Present 1H , 1I
TS21: vomeronasal organ epithelium neuron Present 1J , 1H
Specimen 1K: embryonic day 14.5 (more )
Note: Coronal section. Triple labeled: green - Tubb3; red - Sox10 (assay MGI:7427751); blue - Ngfr (assay MGI:7427800).
Structure Cell Type Level Pattern Image Note
TS22: main olfactory bulb neuron Present 1K
TS22: olfactory epithelium neuron Present 1K , 1L
TS22: olfactory nerve neuron Present 1K , 1L
Specimen 1M: embryonic day 16.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; purple - Sox10 (assay MGI:7427749).
Structure Cell Type Level Pattern Image Note
TS24: olfactory epithelium neuron Present 1M1 , 1M
Specimen 1N: embryonic day 17.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; purple - Sox10 (assay MGI:7427749).
Structure Cell Type Level Pattern Image Note
TS25: olfactory epithelium neuron Present 1N1 , 1N
Specimen 1O: postnatal newborn (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Sox10 (assay MGI:7427751).
Structure Cell Type Level Pattern Image Note
TS27: olfactory epithelium neuron Present 1O1 , 1O
Specimen 2A: embryonic day 11.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Parasagittal section. Double labeled: green - Tubb3; magenta - Sox10 reporter (assay MGI:7427801).
Structure Cell Type Level Pattern Image Note
TS20: olfactory nerve Present 2A1 , 2A2 , 2A , 2A3
TS20: forebrain neuron of the forebrain Present 2A
Specimen 2B: embryonic day 12.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Parasagittal section. Double labeled: green - Tubb3; magenta - Sox10 reporter (assay MGI:7427801).
Structure Cell Type Level Pattern Image Note
TS21: olfactory nerve Present 2B , 2B1
TS21: forebrain neuron of the forebrain Present 2B , 2B1
Specimen 2C: embryonic day 16.5; Sox10tm1Weg/Sox10+ (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Sox10 reporter (assay MGI:7427801).
Structure Cell Type Level Pattern Image Note
TS24: main olfactory bulb neuron Present 2C , 2C2
TS24: main olfactory bulb olfactory nerve layer neuron Present 2C , 2C2
TS24: olfactory nerve Present 2C , 2C2
TS24: nasal cavity lamina propria neuron Present 2C1 , 2C
TS24: olfactory epithelium neuron Present 2C1 , 2C
Specimen 2D: embryonic day 16.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Sox10 reporter (assay MGI:7427801).
Structure Cell Type Level Pattern Image Note
TS24: main olfactory bulb neuron Present 2D
TS24: main olfactory bulb olfactory nerve layer neuron Present 2D2 , 2D
TS24: olfactory nerve Present 2D2 , 2D , 2D1
TS24: nasal cavity lamina propria neuron Present 2D , 2D1
TS24: olfactory epithelium neuron Present 2D , 2D1
Specimen 2E: embryonic day 16.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - S100b (assay MGI:7427799).
Structure Cell Type Level Pattern Image Note
TS24: main olfactory bulb neuron Present 2E
TS24: main olfactory bulb olfactory nerve layer neuron Present 2E , 2E2
TS24: olfactory nerve Present 2E , 2E2
TS24: nasal cavity lamina propria neuron Present 2E , 2E1
TS24: olfactory epithelium neuron Present 2E , 2E1
Specimen 2F: embryonic day 16.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - S100b (assay MGI:7427799).
Structure Cell Type Level Pattern Image Note
TS24: main olfactory bulb neuron of the forebrain Present 2F
TS24: main olfactory bulb olfactory nerve layer neuron Present 2F2 , 2F
TS24: olfactory nerve Present 2F1 , 2F
Specimen 2G: embryonic day 16.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Ngfr (assay MGI:7427800).
Structure Cell Type Level Pattern Image Note
TS24: main olfactory bulb neuron Present 2G
TS24: olfactory nerve Present 2G2 , 2G
TS24: nasal cavity lamina propria neuron Present 2G , 2G1
TS24: olfactory epithelium neuron Present 2G , 2G1
Specimen 2H: embryonic day 16.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Ngfr (assay MGI:7427800).
Structure Cell Type Level Pattern Image Note
TS24: main olfactory bulb neuron Present 2H
TS24: main olfactory bulb olfactory nerve layer neuron Present 2H , 2H2
TS24: olfactory nerve Present 2H , 2H1
TS24: nasal cavity lamina propria neuron Present 2H1 , 2H
TS24: olfactory epithelium neuron Present 2H1 , 2H
Specimen 4C: embryonic day 16.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Omp (assay MGI:7427765).
Structure Cell Type Level Pattern Image Note
TS24: olfactory epithelium neuron Present 4C
Specimen 4D: embryonic day 16.5; Sox10tm1Weg/Sox10+ (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Omp (assay MGI:7427765).
Structure Cell Type Level Pattern Image Note
TS24: olfactory epithelium neuron Present 4D The number of neurons in the heterozygote are similar to those seen in wild type.
Specimen 4E: embryonic day 16.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Omp (assay MGI:7427765).
Structure Cell Type Level Pattern Image Note
TS24: olfactory epithelium neuron Present 4E The number of neurons in the homozygote are similar to those seen in wild type.
Specimen 5E: embryonic day 16.5 (more )
Note: Coronal section. Double labeled: green - Tubb3; white - Gnrh1 (assay MGI:7427757).
Structure Cell Type Level Pattern Image Note
TS24: hypothalamus neuron of the forebrain Present 5E1 , 5E
Specimen 5F: embryonic day 16.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Coronal section. Double labeled: green - Tubb3; magenta - Gnrh1 (assay MGI:7427757).
Structure Cell Type Level Pattern Image Note
TS24: nasal cavity lamina propria neuron Present 5F1 , 5F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory