Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:199435
Barraud P, et al., Olfactory ensheathing glia are required for embryonic olfactory axon targeting and the migration of gonadotropin-releasing hormone neurons. Biol Open. 2013 Jul 15;2(7):750-9
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7427800
|
|
Gene symbol:
|
Ngfr
|
|
Gene name:
|
nerve growth factor receptor (TNFR superfamily, member 16)
|
|
Antibody:
|
anti-p75NTR
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
|
Results
|
Specimen
1K:
embryonic day 14.5
(more  )
Note: Coronal section. Triple labeled: blue - Ngfr; red - Sox10 (assay MGI:7427751); green - Tubb3 (assay MGI:7427814).
Specimen
1K:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Coronal section. Triple labeled: blue - Ngfr; red - Sox10 (assay MGI:7427751); green - Tubb3 (assay MGI:7427814).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: olfactory nerve |
olfactory ensheathing cell |
Present |
|
1L
,
1K
|
Expression is seen in OECs surrounding olfactory nerve fascicles in the lamina propria.
|
Specimen
2G:
embryonic day 16.5
(more )
Note: Coronal section. Double labeled: magenta - Ngfr; green - Tubb3 (assay MGI:7427814).
Specimen
2G:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Coronal section. Double labeled: magenta - Ngfr; green - Tubb3 (assay MGI:7427814).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: nasal cavity lamina propria |
neural crest cell |
Present |
|
2G
,
2G1
|
Expressing cells are distributed along the olfactory nerve from the lamina propria to the olfactory nerve layer of the olfactory bulb.
|
| TS24: main olfactory bulb olfactory nerve layer |
neural crest cell |
Present |
|
2G
,
2G2
|
Expressing cells are distributed along the olfactory nerve from the lamina propria to the olfactory nerve layer of the olfactory bulb.
|
| TS24: olfactory nerve |
neural crest cell |
Present |
|
2G
,
2G2
|
Expressing cells are distributed along the olfactory nerve from the lamina propria to the olfactory nerve layer of the olfactory bulb.
|
Specimen
2H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ
|
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Coronal section. Double labeled: magenta - Ngfr; green - Tubb3 (assay MGI:7427814).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: nasal cavity lamina propria |
neural crest cell |
Absent |
|
2H
,
2H1
|
Expressing cells are seen along portions of the olfactory nerve but are absent from olfactory nerve fascicles in the lamina propria.
|
| TS24: main olfactory bulb olfactory nerve layer |
neural crest cell |
Present |
|
2H2
,
2H
|
Expressing cells are seen along portions of the olfactory nerve but are absent from olfactory nerve fascicles in the lamina propria.
|
| TS24: olfactory nerve |
neural crest cell |
Present |
|
2H2
,
2H
|
Expressing cells are seen along portions of the olfactory nerve but are absent from olfactory nerve fascicles in the lamina propria.
|
|
Analysis Tools