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Gene Expression Data
Assay Details
Assay
Reference: J:253585 Siletti K, et al., Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11170-E11179
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427530
Gene symbol: Ccdc88c
Gene name: coiled-coil domain containing 88C
Antibody: anti-Daple (A302-951A-T)
Detection system: Secondary antibody
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-DAPLE (sc-137416) MGI:7427458 may have been used in this assay.
Results
Specimen 1E Daple+/+: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 1E Daple+/+ Expression at abneural boundaries of hair cells.
Specimen 1E Daple-/-: postnatal day 0; Ccdc88ctm1(KOMP)Mbp/Ccdc88ctm1(KOMP)Mbp (more )
Structure Level Pattern Image Note
TS27: cochlea Trace Regionally restricted 1E Daple-/- No expression at abneural boundaries of hair cells.
Specimen 8A (top): postnatal day 2 (more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - actin.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8A (top) Expression at level of intercellular junctions.
Specimen 8A (bottom): postnatal day 2 (more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - actin.
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8A (bottom) Expression at level of intercellular junctions.
Specimen 8B (top): postnatal day 1 (more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - ZO-1 (Tjp1; assay MGI:7427542).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8B (top) Expression abuts intercellular junctions.
Specimen 8B (bottom): postnatal day 1 (more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - ZO-1 (Tjp1; assay MGI:7427542).
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 8B (bottom) Expression abuts intercellular junctions.
Specimen 8D Apex: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
Structure Cell Type Level Pattern Image Note
TS24: cochlea cochlea auditory hair cell Present Regionally restricted 8D Apex Merged , 8D Apex Daple Expression in many immature hair cells near cochlear apex. In some cells, expression is not assymmetrically distributed; in other cells, expression at neural edge.
Specimen 8D Middle: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
Structure Cell Type Level Pattern Image Note
TS24: cochlea cochlea auditory hair cell Present Regionally restricted 8D Middle Merged , 8D Middle Daple In middle of cochlea, expression confined to abneural halves of some hair cells.
Specimen 8D Base: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
Structure Level Pattern Image Note
TS24: cochlea Present Regionally restricted 8D Base Daple , 8D Base Merged Near base of cochlea, expression domains expanded in surface area.
Specimen 8E Apex: embryonic day 16.5 (more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - Par-3 (Pard3 assay MGI:7427543).
Structure Cell Type Level Pattern Image Note
TS24: cochlea cochlear outer hair cell Present Regionally restricted 8E Apex Merged , 8E Apex Daple Expression enriched at neural edges of several immature outer hair cells at apex of cochlea.
Specimen 8E Base: embryonic day 16.5 (more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - Par-3 (Pard3 assay MGI:7427543).
Structure Cell Type Level Pattern Image Note
TS24: cochlea cochlea auditory hair cell Present Regionally restricted 8E Base Daple , 8E Base Merged Near base of cochlea, expression distributed abneurally in more mature hair cells.
Specimen 9D LGN DEL/+: postnatal day 1; Gpsm2tm1a(EUCOMM)Wtsi/Gpsm2+ (more )
Structure Level Pattern Image Note
TS27: cochlea Present 9D LGN DEL/+
Specimen 9D LGN DEL/DEL: postnatal day 1; Gpsm2tm1a(EUCOMM)Wtsi/Gpsm2tm1a(EUCOMM)Wtsi (more )
Structure Level Pattern Image Note
TS27: cochlea Present Regionally restricted 9D LGN DEL/DEL No expression at abneural junctions of hair cells. Expression enriched near base of kinocilia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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