Gene Expression Data
Assay Details
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Assay
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Reference:
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J:253585
Siletti K, et al., Daple coordinates organ-wide and cell-intrinsic polarity to pattern inner-ear hair bundles. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):E11170-E11179
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7427530
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Gene symbol:
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Ccdc88c
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Gene name:
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coiled-coil domain containing 88C
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Antibody:
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anti-Daple (A302-951A-T)
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Detection system:
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Secondary antibody
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-DAPLE (sc-137416) MGI:7427458 may have been used in this assay.
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Results
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Specimen
1E Daple+/+:
postnatal day 0
(more  )
Specimen
1E Daple+/+:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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optical section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
1E Daple+/+
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Expression at abneural boundaries of hair cells.
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Specimen
1E Daple-/-:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Trace |
Regionally restricted |
1E Daple-/-
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No expression at abneural boundaries of hair cells.
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Specimen
8A (top):
postnatal day 2
(more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - actin.
Specimen
8A (top):
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
8A (top)
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Expression at level of intercellular junctions.
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Specimen
8A (bottom):
postnatal day 2
(more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - actin.
Specimen
8A (bottom):
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - actin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
8A (bottom)
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Expression at level of intercellular junctions.
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Specimen
8B (top):
postnatal day 1
(more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - ZO-1 (Tjp1; assay MGI:7427542).
Specimen
8B (top):
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Double labeled: magenta - Daple (Ccdc88c); green - ZO-1 (Tjp1; assay MGI:7427542).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
8B (top)
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Expression abuts intercellular junctions.
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Specimen
8B (bottom):
postnatal day 1
(more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - ZO-1 (Tjp1; assay MGI:7427542).
Specimen
8B (bottom):
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Double labeled: magenta - Daple (Ccdc88c); green - ZO-1 (Tjp1; assay MGI:7427542).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
8B (bottom)
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Expression abuts intercellular junctions.
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Specimen
8D Apex:
embryonic day 16.5
(more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
Specimen
8D Apex:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
8D Apex Merged
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8D Apex Daple
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Expression in many immature hair cells near cochlear apex. In some cells, expression is not assymmetrically distributed; in other cells, expression at neural edge.
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Specimen
8D Middle:
embryonic day 16.5
(more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
Specimen
8D Middle:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
8D Middle Merged
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8D Middle Daple
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In middle of cochlea, expression confined to abneural halves of some hair cells.
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Specimen
8D Base:
embryonic day 16.5
(more )
Note: Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
Specimen
8D Base:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Triple labeled: magenta - Daple (Ccdc88c); green - Gnai3 (assay MGI:7427532); blue - tubulin.
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Specimen
8E Apex:
embryonic day 16.5
(more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - Par-3 (Pard3 assay MGI:7427543).
Specimen
8E Apex:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Double labeled: magenta - Daple (Ccdc88c); green - Par-3 (Pard3 assay MGI:7427543).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
cochlear outer hair cell |
Present |
Regionally restricted |
8E Apex Merged
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8E Apex Daple
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Expression enriched at neural edges of several immature outer hair cells at apex of cochlea.
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Specimen
8E Base:
embryonic day 16.5
(more )
Note: Double labeled: magenta - Daple (Ccdc88c); green - Par-3 (Pard3 assay MGI:7427543).
Specimen
8E Base:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Double labeled: magenta - Daple (Ccdc88c); green - Par-3 (Pard3 assay MGI:7427543).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
8E Base Daple
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8E Base Merged
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Near base of cochlea, expression distributed abneurally in more mature hair cells.
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Specimen
9D LGN DEL/+:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Gpsm2tm1a(EUCOMM)Wtsi/Gpsm2+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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optical section
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Specimen
9D LGN DEL/DEL:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
9D LGN DEL/DEL
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No expression at abneural junctions of hair cells. Expression enriched near base of kinocilia.
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