Gene Expression Data
Assay Details
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Assay
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Reference:
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J:319706
Silachev D, et al., Mouse models characterize GNAO1 encephalopathy as a neurodevelopmental disorder leading to motor anomalies: from a severe G203R to a milder C215Y mutation. Acta Neuropathol Commun. 2022 Jan 28;10(1):9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7425395
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Gene symbol:
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Bcl11b
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Gene name:
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B cell leukemia/lymphoma 11B
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Results
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Specimen
3A:
embryonic day 18.5
(more  )
Note: Double labeled: green - Bcl11b; red - Tbr1 (assay MGI:7425393).
Specimen
3A:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Bcl11b; red - Tbr1 (assay MGI:7425393).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
pyramidal neuron |
Present |
Regionally restricted |
3A Ctip2
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3A Merged
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Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex.
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Specimen
3B:
embryonic day 18.5; Gnao1em2Katv/Gnao1 +
(more )
Note: Double labeled: green - Bcl11b; red - Tbr1 (assay MGI:7425393).
Specimen
3B:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gnao1em2Katv/Gnao1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Bcl11b; red - Tbr1 (assay MGI:7425393).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
pyramidal neuron |
Present |
Regionally restricted |
3B Ctip2
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3B Merged
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Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant difference in immunopositive cell number was noted compared with wild type.
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Specimen
3C:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gnao1em2Katv/Gnao1em2Katv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Bcl11b; red - Tbr1 (assay MGI:7425393).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
pyramidal neuron |
Present |
Regionally restricted |
3C Ctip2
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3C Merged
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Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant difference in immunopositive cell number was noted compared with wild type.
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Specimen
S2A +/+:
embryonic day 18.5
(more )
Specimen
S2A +/+:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: corpus striatum |
medium spiny neuron |
Present |
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S2A left
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Expression was detected in GABAergic medium-sized spiny neurons.
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Specimen
S2A C215Y/+:
embryonic day 18.5; Gnao1em2Katv/Gnao1 +
(more )
Specimen
S2A C215Y/+:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gnao1em2Katv/Gnao1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: corpus striatum |
medium spiny neuron |
Present |
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S2A center
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No differences were noted in the staining in the striatum.
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Specimen
S2A C215Y/C215Y:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gnao1em2Katv/Gnao1em2Katv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: corpus striatum |
medium spiny neuron |
Present |
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S2A right
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No differences were noted in the staining in the striatum.
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Specimen
S6C +/+:
embryonic day 18.5
(more )
Specimen
S6C +/+:
(close )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
pyramidal neuron |
Present |
Regionally restricted |
S6C +/+ Ctip2
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Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex.
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Specimen
S6C G203R/+:
embryonic day 18.5; Gnao1em1Katv/Gnao1 +
(more )
Specimen
S6C G203R/+:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gnao1em1Katv/Gnao1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
pyramidal neuron |
Present |
Regionally restricted |
S6C G203R/+ Ctip2
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Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant changes in immunopositive cell number was noted compared with wild type.
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Specimen
S6C G203R/G203R:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Gnao1em1Katv/Gnao1em1Katv
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: frontal cortex |
pyramidal neuron |
Present |
Regionally restricted |
S6C G203R/G203R Ctip2
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Expression was detected in projection neurons (strong in layer V and weak in layer VI) in the motor cortex. No significant changes in immunopositive cell number was noted compared with wild type.
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Analysis Tools