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Gene Expression Data
Assay Details
Assay
Reference: J:319706 Silachev D, et al., Mouse models characterize GNAO1 encephalopathy as a neurodevelopmental disorder leading to motor anomalies: from a severe G203R to a milder C215Y mutation. Acta Neuropathol Commun. 2022 Jan 28;10(1):9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7425393
Gene symbol: Tbr1
Gene name: T-box brain transcription factor 1
Antibody: anti-Tbr1 (AB31940)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3A: embryonic day 18.5 (more )
Note: Double labeled: red - Tbr1; green - Bcl11b (assay MGI:7425395).
Structure Cell Type Level Pattern Image Note
TS26: frontal cortex pyramidal neuron Present Regionally restricted 3A Merged , 3A Tbr1 Expression was detected in layer VI projection neurons in the motor cortex.
Specimen 3B: embryonic day 18.5; Gnao1em2Katv/Gnao1+ (more )
Note: Double labeled: red - Tbr1; green - Bcl11b (assay MGI:7425395).
Structure Cell Type Level Pattern Image Note
TS26: frontal cortex pyramidal neuron Present Regionally restricted 3B Tbr1 , 3B Merged Expression was detected in layer VI projection neurons in the motor cortex. A significant decrease in immunopositive cell density was noted compared with wild type.
Specimen 3C: embryonic day 18.5; Gnao1em2Katv/Gnao1em2Katv (more )
Note: Double labeled: red - Tbr1; green - Bcl11b (assay MGI:7425395).
Structure Cell Type Level Pattern Image Note
TS26: frontal cortex pyramidal neuron Present Regionally restricted 3C Tbr1 , 3C Merged Expression was detected in layer VI projection neurons in the motor cortex. A significant decrease in immunopositive cell density was noted compared with wild type.
Specimen S6C +/+: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C +/+ Tbr1 Expression was detected in layer VI in the motor cortex.
Specimen S6C G203R/+: embryonic day 18.5; Gnao1em1Katv/Gnao1+ (more )
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C G203R/+ Tbr1 The Tbr1-positive cells strongly decreased in number compared with wild type in layer VI in the motor cortex.
Specimen S6C G203R/G203R: embryonic day 18.5; Gnao1em1Katv/Gnao1em1Katv (more )
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C G203R/G203R Tbr1 The Tbr1-positive cells strongly decreased in number compared with wild type in layer VI in the motor cortex.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory