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Gene Expression Data
Assay Details
Assay
Reference: J:304809 Muha V, et al., Loss of O-GlcNAcase catalytic activity leads to defects in mouse embryogenesis. J Biol Chem. 2021 Feb 18;:100439
Assay type: RT-PCR
MGI Accession ID: MGI:7425140
Gene symbol: Ogt
Gene name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Probe: Ogt-pE, Ogt-pF
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh, Actb, and Pgk1.
Results
Image: 4D Ogt ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Brain +/+ E15.5 TS23: brain Present 1 µg; total RNA involves: C57BL/6NTac Not Specified
Brain D285A/+ E15.5 TS23: brain Present (a) 1 µg; total RNA involves: C57BL/6NTac Ogatm1.1Arte/Oga+Not Specified
Brain D285A/D285A E15.5 TS23: brain Present (b) 1 µg; total RNA involves: C57BL/6NTac Ogatm1.1Arte/Ogatm1.1ArteNot Specified
Liver +/+ E15.5 TS23: liver Present 1 µg; total RNA involves: C57BL/6NTac Not Specified
Liver D285A/+ E15.5 TS23: liver Present (c) 1 µg; total RNA involves: C57BL/6NTac Ogatm1.1Arte/Oga+Not Specified
Liver D285A/D285A E15.5 TS23: liver Present (c) 1 µg; total RNA involves: C57BL/6NTac Ogatm1.1Arte/Oga+Not Specified
Notes:
(a) Decreased expression (0.6 0.2 fold change) compared with the wild-type, but did not reach statistical significance.
(b) Decreased expression (0.5 0.1 fold change) compared with the wild-type.
(c) Similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory