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Gene Expression Data
Assay Details
Assay
Reference: J:291970 Takemoto K, et al., Meiosis-Specific C19orf57/4930432K21Rik/BRME1 Modulates Localization of RAD51 and DMC1 to DSBs in Mouse Meiotic Recombination. Cell Rep. 2020 May 26;31(8):107686
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7413855
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: anti-Sycp3
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Sycp3 MGI:7413230 may have been used in this assay.
Results
Specimen 2E WT p18: postnatal day 18 (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:7413853).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2E WT (p18)
Specimen 2E Brme1 KO p18: postnatal day 18; Brme1em1Keish/Brme1em1Keish (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:7413853).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2E KO (p18)
Specimen 2E WT 8w: postnatal week 8 (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:7413853).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2E WT (8w)
Specimen 2E Brme1 KO 8w: postnatal week 8; Brme1em1Keish/Brme1em1Keish (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:7413853).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2E KO (8w)
Specimen 2J WT (upper): postnatal day 18 (more )
Note: Double labeled: red - Sycp3; green - TUNEL.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2J WT (upper)
Specimen 2J KO (upper): postnatal day 18; Brme1em1Keish/Brme1em1Keish (more )
Note: Double labeled: red - Sycp3; green - TUNEL.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2J KO (upper)
Specimen 2K WT (upper): postnatal week 8 (more )
Note: Double labeled: red - Sycp3; green - TUNEL.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2K WT (upper)
Specimen 2K KO (upper): postnatal week 8; Brme1em1Keish/Brme1em1Keish (more )
Note: Double labeled: red - Sycp3; green - TUNEL.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 2K KO (upper)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory