Mir29c RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:148942 Vasileva A, et al., Tdrd6 is required for spermiogenesis, chromatoid body architecture, and regulation of miRNA expression. Curr Biol. 2009 Apr 28;19(8):630-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:7387044
Gene symbol:	Mir29c
Gene name:	microRNA 29c

Probe:	Pre-miR-29c-F, Pre-miR-29c-R
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

Image: S7_pre-miR-29c

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
pri-miR-29c	Other lane(s) in blot use different probe(s)
pre-miR-29c +/-	P18	TS28: testis	Present	1 µg; total RNA	either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	Tdrd6^tm1Jess/Tdrd6⁺	Male
pre-miR-29c -/-	P18	TS28: testis	Present (a)	1 µg; total RNA	either: (involves: 129S6/SvEvTac * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	Tdrd6^tm1Jess/Tdrd6^tm1Jess	Male
mature miR-29c	Other lane(s) in blot use different probe(s)
-	Other lane(s) in blot assay different gene(s)

Notes:
(a) There was a significant increase in expression in this mutant compared to heterozygotes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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