Gene Expression Data
Assay Details
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Assay
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Reference:
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J:301026
Bogolyubova IO, et al., The dynamics of DAXX protein distribution in the nucleus of mouse early embryos. Acta Histochem. 2019 May;121(4):522-529
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7384973
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Gene symbol:
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Atrx
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Gene name:
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ATRX, chromatin remodeler
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Results
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Specimen
4a:
embryonic day 0.5
(more  )
Note: late zygote. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
Specimen
4a:
(close  )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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late zygote. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Ambiguous |
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4a''
,
4a'''
,
4a'
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There was no colocalization of Atrx with Daxx immunolabeling at the late zygote stage.
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Specimen
4b:
embryonic day 1.0
(more )
Note: two cell stage. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
Specimen
4b:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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two cell stage. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
4b'''
,
4b''
,
4b'
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At late two-cell stage, colocalization of Daxx and ATRX was detectable both in the ring-shaped heterochromatin zones associated with the nucleolus precursor body (NPB) periphery and in NPB-unassociated heterochromatin patches. Some zones of Daxx localization did not overlap with ATRX (and vise versa). ATRX was found in 60% Daxx-positive heterochromatin areas associated with NPBs and in 80% Daxx-positive heterochromatin clumps located outside the NPBs.
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Specimen
4c:
embryonic day 3.0
(more )
Note: morula stage. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
Specimen
4c:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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morula stage. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Ambiguous |
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4c''
,
4c'
,
4c'''
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There was no colocalization of Atrx with Daxx immunolabeling at the morula stage.
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Specimen
5a:
embryonic day 1.0
(more )
Note: two cell stage. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
Specimen
5a:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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two cell stage. Double labeled: green - Atrx; red - Daxx (assay MGI:7384958).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
5a''
,
5a'
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Daxx and Atrx colocalization in the nucleus of 2-cell mouse embryos.
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Analysis Tools